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ZFIN ID: ZDB-FIG-110624-3
Row et al., 2011 - Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail. Developmental Biology   354(1):102-110 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Prim-25

Fig. 4

Restoring Spadetail significantly rescues the spt phenotypes. (A) A genetic construct was incorporated into the zebrafish genome by Tol2-mediated transgenesis. The spt and eGFP genes were placed downstream of a bi-directional heat shock-sensitive promoter. (B) Expressing spt from this construct partially restored expression of tbx24 in spt morphant embryos. (C) Significant phenotypic rescue was also observed by inducing transgene expression early in gastrulation. Arrowheads mark the position of the first and last formed somite in each embryo.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
w72Tg + MO1-tbx16 + MO2-tbx16(WIK/AB) heat shock Prim-25 somite decreased amount, abnormal
Prim-25 somite development disrupted, abnormal
w72Tg + MO1-tbx16 + MO2-tbx16(WIK/AB) standard conditions Prim-25 somite decreased amount, abnormal
Prim-25 somite development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 354(1), Row, R.H., MaƮtre, J.L., Martin, B.L., Stockinger, P., Heisenberg, C.P., and Kimelman, D., Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail, 102-110, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.