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ZFIN ID: ZDB-FIG-110624-2
Row et al., 2011 - Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail. Developmental Biology   354(1):102-110 Full text @ Dev. Biol.
Knockdown Reagents:
Observed In:
Stage Range: Germ-ring to Shield

Fig. 2

Mesoderm in spt morphants displays abnormal morphology. (A) Cells in the hypoblast of spt morphant embryos blebbed extensively and failed to adopt the proper mesenchymal morphology. (B) Cell protrusions were quantified in wild-type embryos during and after involution, in the hypoblast of spt morphants and in spt morphants expressing spt under the control of a heat shock-sensitive promoter. Mesodermal cells in spt morphants blebbed far more than in wild-type hypoblast, whereas restoring Spadetail significantly reduced this blebbing. Error bars indicate one standard deviation. At least 22 cells were quantified per condition. p < 0.05 for data marked with an asterisk.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tbx16 + MO2-tbx16 standard conditions Germ-ring - Shield hypoblast bleb increased amount, abnormal
Germ-ring - Shield mesoderm morphology, abnormal
Germ-ring - Shield mesodermal to mesenchymal transition involved in gastrulation disrupted, abnormal
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 354(1), Row, R.H., MaƮtre, J.L., Martin, B.L., Stockinger, P., Heisenberg, C.P., and Kimelman, D., Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail, 102-110, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.