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ZFIN ID: ZDB-FIG-110624-4
Row et al., 2011 - Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail. Developmental Biology   354(1):102-110 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Germ-ring to Shield

Fig. 5

Cell morphology and cell migration are rescued by restoring Spadetail. (A) Cells in the hypoblast of spt morphant cells expressing spt after transgene induction blebbed less frequently than with morpholino alone (see Fig. 2B for quantification). (B) When spt morphant cells were transplanted into the ventral mesoderm of a wild-type embryo at shield stage they were unable to contribute to somites. (C) Restoring Spadetail by inducing transgene expression allowed cells to migrate out of the tailbud and contribute to developing somites. Each panel is representative of at least 16 successful transplants.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
w72Tg + MO1-tbx16 + MO2-tbx16(WIK/AB) heat shock Germ-ring - Shield hypoblast bleb increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 354(1), Row, R.H., MaƮtre, J.L., Martin, B.L., Stockinger, P., Heisenberg, C.P., and Kimelman, D., Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail, 102-110, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.