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ZIRC
ZFIN ID: ZDB-FIG-110428-11
Bussmann et al., 2011 - Arterial-venous network formation during brain vascularization involves hemodynamic regulation of chemokine signaling. Development (Cambridge, England)   138(9):1717-1726 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-25 to Pec-fin
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Prim-25 to Pec-fin

Fig. 2

VegfA and Notch signaling are required for angiogenic sprouting in the zebrafish hindbrain. (A-D) Whole-mount in situ hybridization showing the expression of vegfaa (A), vegfab (B), kdrl (C) and dll4 (D) at 36 hpf. (E-L) Maximal intensity projections of confocal z-stacks at 36 (E,F,K,L), 54 (I,J) or 60 (G,H) hpf. Arrowheads indicate CtA sprouts emerging from the PHBC. Arrow indicates the position of the BA. (E,F) Sibling (E) or kdrl mutant (F) embryos, with endothelial cells labeled by kdrl:mem-rfp expression. (G,H) kdrl:mem-rfp-expressing embryos injected with control MO (G) or dll4 MO (H). (I,J) kdrl:gfp-expressing embryos treated from 30 to 54 hpf with DMSO (I) or DAPT (J). (K,L) Embryos from a cross between cdh5:gal4ff, uas:gfp and uas:NICD. The embryo in K is negative for uas:NICD, whereas that in L is heterozygous for uas:NICD. Endothelial cells are labeled by cdh5:gal4ff, uas:gfp expression. Dorsal views, anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dll4 WT standard conditions Prim-25 brain vasculature ISH
EGFP kca3Tg; mu101Tg; nkuasgfp1aTg standard conditions Prim-25 primordial hindbrain channel blood vessel endothelium IFL
mu101Tg; nkuasgfp1aTg standard conditions Prim-25 basilar artery blood vessel endothelium IFL
Prim-25 primordial hindbrain channel blood vessel endothelium IFL
s843Tg control Long-pec basilar artery blood vessel endothelium IFL
Long-pec central artery blood vessel endothelium IFL
Long-pec primordial hindbrain channel blood vessel endothelium IFL
s843Tg chemical treatment: DAPT Long-pec basilar artery blood vessel endothelium IFL
Long-pec central artery blood vessel endothelium IFL
Long-pec primordial hindbrain channel blood vessel endothelium IFL
kdrl WT standard conditions Prim-25 primordial hindbrain channel blood vessel endothelium ISH
mCherry kdrlhu5088/hu5088; s916Tg standard conditions Prim-25 Not Detected basilar artery IFL
Prim-25 primordial hindbrain channel blood vessel endothelium IFL
s916Tg control Prim-25 basilar artery blood vessel endothelium IFL
Prim-25 primordial hindbrain channel blood vessel endothelium IFL
Pec-fin basilar artery blood vessel endothelium IFL
Pec-fin central artery blood vessel endothelium IFL
Pec-fin primordial hindbrain channel blood vessel endothelium IFL
s916Tg + MO2-dll4 standard conditions Pec-fin basilar artery blood vessel endothelium IFL
Pec-fin central artery blood vessel endothelium IFL
Pec-fin primordial hindbrain channel blood vessel endothelium IFL
vegfaa WT standard conditions Prim-25 hindbrain ISH
vegfab WT standard conditions Prim-25 hindbrain floor plate ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kca3Tg; mu101Tg; nkuasgfp1aTg standard conditions Prim-25 basilar artery physical object quality, abnormal
Prim-25 blood vessel endothelial cell decreased amount, abnormal
Prim-25 central artery decreased amount, abnormal
Prim-25 sprouting angiogenesis decreased occurrence, abnormal
kdrlhu5088/hu5088; s916Tg standard conditions Prim-25 basilar artery absent, abnormal
Prim-25 central artery absent, abnormal
Prim-25 sprouting angiogenesis decreased occurrence, abnormal
s843Tg chemical treatment: DAPT Long-pec central artery increased amount, abnormal
Long-pec sprouting angiogenesis increased occurrence, abnormal
s916Tg + MO2-dll4 standard conditions Pec-fin central artery increased amount, abnormal
Pec-fin sprouting angiogenesis increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development