ZFIN ID: ZDB-FIG-110112-11
Schwend et al., 2010 - Visualization of gli activity in craniofacial tissues of hedgehog-pathway reporter transgenic zebrafish. PLoS One   5(12):e14396 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: mCherry, shha
Fish: WT, nu15Tg
Anatomical Terms: axial mesoderm, blastoderm, brain, diencephalon, floor plate (all 15) expand
Stage Range: Germ-ring to Day 4

Fig. 3 mCherry reporter gene and protein expression reflects known Hh-expressing domains in Tg(Gli-d:mCherry).

Lateral views (A,B,D,E,G,H,J,K,L,L2,M,N) or dorsal views (C′,C",F,I,M′), anterior to the left, of Tg(Gli-d:mCherry) fish labeled with mCherry (A–F,K,L,M,N) or shh (G) riboprobes or mCherry protein which was visualized by confocal microscopy (H–J,L′). (A) mCherry is broadly expressed in blastomeres at 60% epiboly. (B–C") By 11 hpf, mCherry was localized to the neural plate (np) and the midline notochord (no) tissues. (D) At 17 hpf, mCherry protein was apparent in forebrain (fb) and floor plate (fp). (E–G) At 22–24 hpf, mCherry gene (E,F) and protein (H–J) are expressed in the fb, midbrain and fp, similar to shh gene expression (G) at a comparable stage. (K–N) At later stages from 2 to 4 dpf, mCherry is shown in whole-embryo lateral views and detectable in brain, fp, myotomal adaxial cells and PA. (L′) Confocal projections of Mcherry protein corresponding to boxed region in panel L revealed expression in the adaxial cells. (M′) Dorsal view of boxed region in panel M showed mCherry expression in adaxial cells and in somitic tissue neighboring the notochord in an orderly, segmental fashion. Abbreviations: di, diencephalon; fb, forebrain; fmb, forebrain-midbrain boundary; fp, floor plate; hb, hindbrain; mb, midbrain; np, neural plate; no, notochord; os, optic stalk; ov, otic vesicle; PA, pharyngeal arches; pcp, prechordal plate; te, telencephalon.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry nu15Tg standard conditions Germ-ring blastoderm ISH
1-4 somites axial mesoderm ISH
1-4 somites neural plate ISH
1-4 somites notochord ISH
14-19 somites forebrain IFL
26+ somites axial mesoderm ISH
26+ somites diencephalon ISH
26+ somites floor plate ISH
26+ somites forebrain ISH
26+ somites forebrain midbrain boundary ISH
26+ somites optic stalk ISH
26+ somites otic vesicle ISH
Prim-5 axial mesoderm IFL
Prim-5 diencephalon IFL
Prim-5 floor plate IFL
Prim-5 forebrain midbrain boundary IFL
Prim-5 optic stalk IFL
Prim-15 floor plate ISH
Prim-15 forebrain ISH
Prim-15 forebrain midbrain boundary ISH
Long-pec brain ISH
Long-pec floor plate ISH
Long-pec myotome adaxial cell ISH
Long-pec pharyngeal arch ISH
Protruding-mouth brain ISH
Protruding-mouth myotome ISH
Protruding-mouth pharyngeal arch ISH
Day 4 brain ISH
Day 4 myotome ISH
Day 4 pharyngeal arch ISH
shha WT standard conditions Prim-15 floor plate ISH
Prim-15 forebrain ISH
Prim-15 forebrain midbrain boundary ISH
Prim-15 midbrain ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One