ZFIN ID: ZDB-FIG-110112-13
Schwend et al., 2010 - Visualization of gli activity in craniofacial tissues of hedgehog-pathway reporter transgenic zebrafish. PLoS One   5(12):e14396 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: mCherry, sox9a
Fish: WT, nu15Tg
Anatomical Terms: brain ventral region, ceratobranchial cartilage, cranial nerve II, mandibular arch skeleton, Meckel's cartilage (all 10) expand
Stage Range: Prim-15 to Day 4

Fig. 5 Reporter expression in Tg(Gli-d:mCherry) craniofacial region during embryogenesis and larval stages.

Lateral (A–M) or ventral (N–Q) views, anterior to the left, of 33 hpf (A–C), 38 hpf (D–F), 48 hpf (G–I), 52 hpf (J), 60 hpf (K), 72 hpf (L–O) or 4.5 dpf (P,Q) transgenic fish stained with mCherry riboprobe (A,D,G,J,K,L,N,P) or sox9a riboprobe (M,O) for gene expression or Alcian blue (Q) to visualize cartilage elements. Confocal stack projections of Tg(Gli-d:mCherry) and Tg(Fli1:gfp) double transgenic fish (B,C,E,F,H,I). In these fish, reporter (red) and neural crest cells (green) can be visualized simultanenously. Of note, in (H–I) the reporter-positive pe tissue separated slightly from PA tissue during mounting of embryo. In (I) green-positive neural crest cells are not in the focal plane, despite residing just dorsal to mCherry expressing pe cells. (A–C) At 33 hpf, transgene is expressed in ventral brain and oral ectoderm. (D–F) At 38 hpf, expression persists in brain and ectoderm and expands to pharyngeal endoderm. (G–I) At 48 hpf, expression persists in brain and facial epithelium and expands to mesenchyme in anterior arches. (J–K) From 52–60 hpf, transgene expression expands to mesenchyme in all arches. (L–O) At 72 hpf, transgene expression in PA closely matches the mesenchymal neural crest marker sox9a. (P,Q) At 4.5 dpf, transgene expression persists in each arch and the pattern matches cartilage elements. Abbreviations: bh, basihyal; cb, ceratobranchial; ch, ceratohyal; hs, hyosymplectic; Mc, Meckel′s cartilage; oe, oral ectoderm; on, optic nerve; PA, pharyngeal arch; pe, pharyngeal endoderm; pq, palatoquadrate; tr, trabeculae; vb, ventral brain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry nu15Tg standard conditions Prim-15 brain ventral region IFL
Prim-15 oral region ectoderm IFL
Prim-25 oral region ectoderm IFL
Prim-25 pharyngeal arch endoderm IFL
Long-pec brain ventral region IFL
Long-pec mandibular arch skeleton IFL
Long-pec oral ectoderm IFL
Long-pec pharyngeal arch cranial neural crest cell ISH
Long-pec pharyngeal arch cranial neural crest cell IFL
Long-pec pharyngeal arch endoderm IFL
Protruding-mouth neurocranial trabecula ISH
Protruding-mouth pharyngeal arch cranial neural crest cell ISH
Day 4 ceratobranchial cartilage ISH
Day 4 cranial nerve II ISH
Day 4 Meckel's cartilage ISH
sox9a WT standard conditions Protruding-mouth neurocranial trabecula ISH
Protruding-mouth pharyngeal arch cranial neural crest cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One