ZFIN ID: ZDB-FIG-101011-36
Zanazzi et al., 2010 - Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development. Neural Development   5:24 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Pec-fin to Protruding-mouth

Fig. 5 Complexin 3/4 concentrates in retinal photoreceptor terminals concomitant with RIBEYE b. (A) The zpr 1/FRet 43 monoclonal antibody (red) and the pan-complexin 3/4 polyclonal (green) were also used to localize these complexins in developing retinal photoreceptors. At 2.5 dpf, retinal photoreceptors are most prominent in the ventronasal patch, where complexin 3/4 immunoreactivity appears in some photoreceptor terminals. (B) RIBEYE b (green) has also started to cluster in photoreceptor terminals (red) in the outer plexiform layer at 2.5 dpf. (C) At 3 dpf, complexin 3/4 (red) is highly expressed in zpr 1/FRet 43-positive (red) and zpr 1/FRet 43-negative photoreceptor terminals in the ventronasal patch. (D) Pleiomorphic RIBEYE b (green) expression is found at 3 dpf in zpr 1/FRet 43-positive (red) and zpr 1/FRet 43-negative photoreceptor terminals in the ventronasal patch. The inset shows a zpr 1/FRet 43-positive terminal containing curvilinear RIBEYE b immunoreactivity that may correspond to a ribbon. Scale bar = 5 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-cplx3/4 IHC WT standard conditions Pec-fin photoreceptor cell axon terminus
IHC Protruding-mouth photoreceptor cell axon terminus
IHC Protruding-mouth retina ventral region
Ab1-ctbp2l IHC WT standard conditions Pec-fin photoreceptor cell axon terminus
IHC Protruding-mouth photoreceptor cell axon terminus
IHC Protruding-mouth retina ventral region
zpr-1 IHC WT standard conditions Pec-fin photoreceptor cell
IHC Pec-fin retina ventral region
IHC Protruding-mouth photoreceptor cell
IHC Protruding-mouth retina ventral region
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.