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ZFIN ID: ZDB-FIG-101011-44
Zanazzi et al., 2010 - Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development. Neural Development   5:24 Full text @ Neural Dev.
Knockdown Reagent:
Anatomical Terms:
Stage: Day 4

Fig. 11 A complexin 4a morpholino antisense oligonucleotide knocks down complexin 4a protein expression in the larval zebrafish retina and pineal organ. (A-H) 4.3-dpf control (A, C, E, G) and complexin 4a morpholino-injected (B, D, F, H) larval zebrafish were sectioned transversely and stained with anti-zpr 1/FRet 43 (red) and either anti-complexin 4a (A, B, E, F) or anti-complexin 3/4 (C, D, G, H) (green). The complexin 4a translation-blocking morpholino dramatically decreases complexin 4a expression in the pineal organ (B) and in the retinal outer plexiform layer (OPL) and inner plexiform layer (IPL) (F). Some complexin 3/4 immunoreactivity remains in the pineal (D) and retina (H). Scale bars: 15 μm (A-D); 30 μm (E-H).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cplx4a Ab1-cplx4a WT + MO1-cplx4a standard conditions Day 4 Not Detected epiphysis IHC
Ab1-cplx4a Day 4 Not Detected retinal inner plexiform layer IHC
Ab1-cplx4a Day 4 Not Detected retinal outer plexiform layer IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-cplx3/4 IHC WT + MO1-cplx4a standard conditions Day 4 epiphysis
IHC Day 4 retina
Phenotype Details No data available
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.