ZFIN ID: ZDB-FIG-100706-21
Takada et al., 2010 - Identification of genes expressed by zebrafish oligodendrocytes using a differential microarray screen. Developmental dynamics : an official publication of the American Association of Anatomists   239(7):2041-2047 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth

Fig. 1 HDAC inhibitor TSA blocks oligodendrocyte formation. Embryos were treated with TSA from 36 hours postfertilization (hpf) to 3 days postfertilization (dpf). A,C,E,G,I: Controls. B,D,F,H,J: Trichostatin A (TSA) treated. A-D: Lateral views of the spinal cord. E-J: Transverse sections of the spinal cord of Tg(olig2:egfp) larvae. A,B: Images of the spinal cord of living Tg(olig2:egfp) larvae at 3 dpf. Asterisks indicate dorsally migrated oligodendrocyte progenitor cells (OPCs). C,D: Expression of mbp mRNA at 3 dpf. E,F: Sox10 antibody labeling (red). Arrows indicate Sox10+ olig2:EGFP+ OPCs. G,H: HuC antibody labeling (red) showing neurons. I,J: Zrf-1antibody labeling (red) showing radial glia. Scale bars = 60 μm in A,B, 15 μm in E-J. SC, spinal cord; EGFP, enhanced green fluorescent protein.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mbpa vu12Tg control Protruding-mouth spinal cord oligodendrocyte ISH
vu12Tg chemical treatment: pharmaceutical Protruding-mouth Not Detected spinal cord oligodendrocyte ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC vu12Tg control Protruding-mouth neuron
IHC vu12Tg chemical treatment: pharmaceutical Protruding-mouth neuron
Ab1-sox10 IHC vu12Tg control Protruding-mouth oligodendrocyte
IHC vu12Tg chemical treatment: pharmaceutical Protruding-mouth oligodendrocyte
zrf-1 IHC vu12Tg control Protruding-mouth radial glial cell
IHC vu12Tg chemical treatment: pharmaceutical Protruding-mouth radial glial cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.