PUBLICATION

Identification of genes expressed by zebrafish oligodendrocytes using a differential microarray screen

Authors
Takada, N., and Appel, B.
ID
ZDB-PUB-100621-21
Date
2010
Source
Developmental dynamics : an official publication of the American Association of Anatomists   239(7): 2041-2047 (Journal)
Registered Authors
Appel, Bruce, Takada, Norio
Keywords
myelin, glia, differentiation, RNA profiling
MeSH Terms
  • Animals
  • Embryo, Nonmammalian/metabolism
  • Female
  • Flow Cytometry
  • Gene Expression Regulation, Developmental*/genetics
  • Gene Expression Regulation, Developmental*/physiology
  • Immunohistochemistry
  • In Situ Hybridization
  • Male
  • Myelin Sheath/metabolism
  • Oligodendroglia/cytology*
  • Oligodendroglia/metabolism*
  • Oligonucleotide Array Sequence Analysis
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
PubMed
20549738 Full text @ Dev. Dyn.
Abstract
Myelination of central nervous system axons requires that oligodendrocytes extend multiple membrane processes that specifically recognize and wrap axons, which is followed by expression of proteins necessary for formation of myelin sheaths. To identify new genes that might be important for myelination, we used microarrays to analyze the expression profiles of cells sorted from transgenic zebrafish embryos and larvae under conditions that permitted or blocked oligodendrocyte development. Here, we describe eight genes that have not been previously implicated in oligodendrocyte development. Among the predicted functions of proteins encoded by these genes are lipid sensing, cell-cell junction formation, cytoskeleton regulation, and intracellular signaling. The predicted functions raise the possibility that these genes are involved in multiple cellular events during oligodendrocyte differentiation and myelin formation.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes