FIGURE

Fig. 3

ID
ZDB-FIG-100527-3
Publication
Zhang et al., 2010 - Interaction between thymidylate synthase and its cognate mRNA in zebrafish embryos
Other Figures
All Figure Page
Back to All Figure Page
Fig. 3

Specific interaction between zebrafish TS and its cognate mRNA in vitro.

A gel shift experiment was performed using 32P-labeled full-length TS mRNA as the probe. Zebrafish TS protein (100 ng) was incubated with 32P-labeled full-length TS mRNA (lane 1, arrow). One hundred-fold molar excess luciferase mRNA did not affect the interaction between TS protein and mRNA (lane 2). In lane 3, 100-fold molar excess of unlabeled TS mRNA was included in the incubation buffer. Using bovine serum albumin in place of the TS protein did not result in any specific interaction (lane 4). Lane 5 contained 100 ng 32P-labeled zebrafish TS mRNA only.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One