FIGURE

Fig. 6

ID

ZDB-FIG-100527-5

Publication

Zhang et al., 2010 - Interaction between thymidylate synthase and its cognate mRNA in zebrafish embryos

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Zebrafish TS protein interacted with its own mRNA in vivo.

A, Western blot analysis was performed with purified recombinant zebrafish TS (lane 1) or protein from the immunoprecipitation complex (lane 2) and detected with human TS106 antibody. B, RT-PCR products using the RNA extracted from immunoprecipitation complex as the template. TS mRNA from nt 340–1040 was amplified by RT-PCR using TS-specific primers (lanes 4 and 5). RNA and protein complex was precipitated without TS antibody and RT-PCR was performed (lane 2). Lane 3 indicates the RT-PCR results precipitating the complex with unrelated polyclonal antibody, CHX10-like zebrafish antibody (GenWay Biotech, CA). Lane 1 represents the DNA marker of molecular weight.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One