ZFIN Image: Zhang et al., 2010, Fig. 3

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Figure Caption

Fig. 3 Specific interaction between zebrafish TS and its cognate mRNA in vitro.

A gel shift experiment was performed using ³²P-labeled full-length TS mRNA as the probe. Zebrafish TS protein (100 ng) was incubated with ³²P-labeled full-length TS mRNA (lane 1, arrow). One hundred-fold molar excess luciferase mRNA did not affect the interaction between TS protein and mRNA (lane 2). In lane 3, 100-fold molar excess of unlabeled TS mRNA was included in the incubation buffer. Using bovine serum albumin in place of the TS protein did not result in any specific interaction (lane 4). Lane 5 contained 100 ng ³²P-labeled zebrafish TS mRNA only.

Acknowledgments

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