ZFIN ID: ZDB-FIG-100304-25
Brown et al., 2010 - Nlcam modulates midline convergence during anterior neural plate morphogenesis. Developmental Biology   339(1):14-25 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 1-4 somites to 5-9 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 1-4 somites

Fig. 6 nlcam loss of function causes delayed convergence. (A–D) At 4SS, a deep cleft at the midline in nlcam ATG-MO injected (0.3 mM) embryos (B) indicates that forebrain cells have not yet converged to the midline, as they have in control embryos (A). This is confirmed by in situ hybridisation against emx3 (A′, B′). This phenotype is transient, as demonstrated by the recovered forebrain morphology by 8SS (C, D). (E–G) Graphs show selected statistics for forebrain cell convergence from a single dataset. (E) Position of cells relative to the midline. (F) MSD along the ML axis. (G) Speed along the ML axis. Blue = wt cells; red = nlcam-MO.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
emx3 WIK/AB standard conditions 1-4 somites forebrain neural keel ISH
5-9 somites forebrain neural rod ISH
WIK/AB + MO1-alcamb standard conditions 1-4 somites forebrain neural keel ISH
5-9 somites forebrain neural rod ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WIK/AB + MO1-alcamb standard conditions 1-4 somites dorsal convergence delayed, abnormal
1-4 somites forebrain cell migration delayed, abnormal
1-4 somites forebrain neural keel physical object quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 339(1), Brown, K.E., Keller, P.J., Ramialison, M., Rembold, M., Stelzer, E.H., Loosli, F., and Wittbrodt, J., Nlcam modulates midline convergence during anterior neural plate morphogenesis, 14-25, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.