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ZFIN ID: ZDB-FIG-090527-23
Phelps et al., 2009 - A two-step model for colon adenoma initiation and progression caused by APC loss. Cell   137(4):623-634 Full text @ Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth

Fig. 6 apc Control of Cellular Fate and Differentiation Are Mediated by ctbp1 and Are β-Catenin Independent

(A) Protein from 48 hpf apcmcr zebrafish embryos injected with either 6 x His-APC955–2075 or 6 x His-APC955–2075-AALP were subjected to western blot analysis for β-catenin (top), ctbp1 (second), 6 x His (third), or vinculin (bottom).

(B) Embryos injected as above were fixed at 72 hpf and subjected to in situ hybridization for i-fabp.

(C) apcmcr embryos were treated with DMSO or NS-398 or injected with ctbp1-directed morpholino in the presence or absence of NS-398. At 72 hpf, the embryos were fixed and subjected to in situ hybridization for i-fabp, NaPi, hoxa13a, or evx1.

All images are representative of at least three independent experiments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
evx1 WT control Protruding-mouth cloaca ISH
apchu745/hu745 control Protruding-mouth intestine ISH
apchu745/hu745 + MO2-ctbp1 standard conditions Protruding-mouth cloaca ISH
fabp2 WT control Protruding-mouth intestine ISH
apchu745/hu745 control Protruding-mouth Not Detected intestine ISH
apchu745/hu745 + MO2-ctbp1 standard conditions Protruding-mouth intestine ISH
hoxa13a WT control Protruding-mouth cloaca ISH
apchu745/hu745 control Protruding-mouth intestine ISH
apchu745/hu745 + MO2-ctbp1 standard conditions Protruding-mouth cloaca ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cell, 137(4), Phelps, R.A., Chidester, S., Dehghanizadeh, S., Phelps, J., Sandoval, I.T., Rai, K., Broadbent, T., Sarkar, S., Burt, R.W., and Jones, D.A., A two-step model for colon adenoma initiation and progression caused by APC loss, 623-634, Copyright (2009) with permission from Elsevier. Full text @ Cell