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ZFIN ID: ZDB-FIG-090527-18
Phelps et al., 2009 - A two-step model for colon adenoma initiation and progression caused by APC loss. Cell   137(4):623-634 Full text @ Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth

Fig. 1 KRAS Promotes Intestinal Cell Proliferation and Nuclear Localization of β-Catenin following Loss of APC

(A) apcmcr zebrafish embryos were injected at the one-cell stage with KRAS mRNA. The 72 hpf embryos were fixed, sectioned, and stained for hematoxylin and eosin (H&E) (row 1, sagital section; row 2, cross section), DNA (magenta, middle and bottom), and either PCNA (middle, green) or β-catenin (bottom, green). Overlapping expression is shown in white.

(B) Protein lysates from 72 hpf embryos were subjected to western blot analysis for either V5-tag (top-left) and total RAS (bottom-left) or phospho-Erk (top-right) and vinculin (bottom-right).

(C) Zebrafish harboring an integrated β-catenin TOPGFP reporter were injected with KRAS mRNA, APC morpholino, or both. The 72 hpf embryos were subjected to whole-mount in situ hybridization for GFP. Boxes indicate the intestine (top), and arrows indicate the hindbrain (bottom).

All images were captured using the same exposure and represent at least three independent experiments. Scale bar, 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP w25Tg control Protruding-mouth hindbrain ISH
Protruding-mouth intestine ISH
w25Tg + MO1-apc standard conditions Protruding-mouth hindbrain ISH
Protruding-mouth Not Detected intestine ISH
pcna Ab2-pcna WT standard conditions Protruding-mouth intestine epithelial cell IHC
Ab2-pcna apchu745/hu745 standard conditions Protruding-mouth intestine epithelial cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-ctnnb IHC WT standard conditions Protruding-mouth intestine epithelial cell
IHC apchu745/hu745 standard conditions Protruding-mouth intestine epithelial cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cell, 137(4), Phelps, R.A., Chidester, S., Dehghanizadeh, S., Phelps, J., Sandoval, I.T., Rai, K., Broadbent, T., Sarkar, S., Burt, R.W., and Jones, D.A., A two-step model for colon adenoma initiation and progression caused by APC loss, 623-634, Copyright (2009) with permission from Elsevier. Full text @ Cell