ZFIN ID: ZDB-FIG-090511-4
Hill et al., 2009 - Potential roles of arnt2 in zebrafish larval development. Zebrafish   6(1):79-91 Full text @ Zebrafish
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig. 4 Sensory neurons involved in the touch and startle responses appear normal in arnt2-/- embryos at 24–30 hpf. Lateral views for whole-mount ISH of islet1 in a representative WT larva and arnt2-/- larva is shown at 24 hpf (A, B). The pattern of islet1 staining in the brain (black arrow) is similar for the two genotypes, suggesting that regions where primary neurons form are developing normally. 3A10 IHC staining of Mauthner neurons is similar in a representative WT embryo and arnt2-/- mutant embryo at 30 hpf (C, D). ZNP1 IHC to identify primary motor neurons and trigeminal ganglia is shown in a lateral view of a WT embryo and an arnt2-/- embryo (E, F) and in a dorsal view (G, H) at 30 hpf. ZNP1 IHC is similar in the two genotypes. Results in all panels are representative of 5–9 embryos/genotype.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
isl1a WT standard conditions Prim-5 primary neuron ISH
Prim-15 primary motor neuron ISH
arnt2hi1715Tg/hi1715Tg standard conditions Prim-5 primary neuron ISH
Prim-15 primary motor neuron ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
znp-1 IHC WT standard conditions Prim-5 primary motor neuron
IHC Prim-5 trigeminal ganglion
IHC Prim-15 primary motor neuron
IHC Prim-15 trigeminal ganglion
IHC arnt2hi1715Tg/hi1715Tg standard conditions Prim-5 primary motor neuron
IHC Prim-5 trigeminal ganglion
IHC Prim-15 primary motor neuron
IHC Prim-15 trigeminal ganglion
Phenotype Details
Fish Conditions Stage Phenotype
arnt2hi1715Tg/hi1715Tg standard conditions Prim-5 Mauthner neuron morphology, normal
Prim-5 primary neuron morphology, normal
Prim-15 Mauthner neuron morphology, normal
Prim-15 primary neuron morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Zebrafish for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Zebrafish