ZFIN ID: ZDB-FIG-090506-17
Rhinn et al., 2009 - Zebrafish gbx1 refines the midbrain-hindbrain boundary border and mediates the Wnt8 posteriorization signal. Neural Development   4:12 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Shield to Bud
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Shield to Bud

Fig. 3 gbx1 acts as a mediator of the Wnt8 hindbrain posteriorizing signal. (A) Control embryo at 24 h, (B) gbx1-injected embryo (200 pg), (C) wnt8-injected embryo (400 pg). gbx1 overexpression mimics wnt8 gain-of-function; the embryos are truncated anteriorly. The red arrowhead indicates the position of the ear. (D-G′) Animal pole views at 60% epiboly after ISH with otx2. (D) Control embryo, (E, E′) gbx1-injected embryos (200 pg), (F) embryo injected with wnt8 morpholinos, (G, G′) embryos co-injected with gbx1 mRNA and wnt8 morpholinos. Expansion of the otx2 domain in the wnt8 morphant is rescued by co-injection with gbx1 mRNA. (H-K′) Dorsal views at the tailbud (tb) stage after ISH with otx2 (blue) and krox20 (red). (H) Control embryo, (I, I′) gbx1-injected embryos, (J) embryo injected with wnt8 morpholinos, (K, K′) embryos co-injected with gbx1 mRNA and wnt8 morpholinos. Loss of krox20 and posterior expansion of the otx2 domain in the wnt8 morphant is rescued by gbx1 mRNA.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
egr2b WT standard conditions Bud presumptive rhombomere 3 ISH
Bud presumptive rhombomere 5 ISH
WT + MO1-wnt8a standard conditions Bud Not Detected presumptive rhombomere 3 ISH
Bud Not Detected presumptive rhombomere 5 ISH
otx2b WT standard conditions Shield presumptive forebrain ISH
Shield presumptive midbrain ISH
Bud forebrain neural keel ISH
Bud midbrain neural keel ISH
WT + MO1-wnt8a standard conditions Shield presumptive forebrain ISH
Shield presumptive midbrain ISH
Bud forebrain neural keel ISH
Bud midbrain neural keel ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-wnt8a standard conditions Shield presumptive neural plate wholly anteriorized, abnormal
Bud neural keel wholly anteriorized, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.