FIGURE

Fig. 5

ID

ZDB-FIG-090506-21

Publication

Rhinn et al., 2009 - Zebrafish gbx1 refines the midbrain-hindbrain boundary border and mediates the Wnt8 posteriorization signal

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Fig. 5

gbx1 morpholinos affect hindbrain patterning. (A-C′) Embryos at the four somite stage stained for otx2/krox20/myod. (A) Control embryo, (B, B′) embryos injected with 10 ng Mo1, (C, C′) embryos injected with 7 ng Mo2. Injections of two different morpholinos show the same phenotype morphologically and after in situ hybridization analysis. (D-F) Rescue experiments using the krox20 expression domain as a read-out. (D) gbx1-morphant, (E, E′) rescued morphant embryos co-injected with gbx1 mRNA that does not contain the MO binding sequence in its 5′ region. (F) Two combinations were tested: 10 ng of Mo1 with 200 pg or 400 pg of gbx1 mRNA. (G, H) Western blot detection showing down regulation of gbx1-myc mRNA translation in embryos co-injected with gbx1-myc mRNA containing morpholino binding sequences and Mo1 (G) or Mo2 (H). WT, wild type.

Expression Data

Genes:	egr2b myod1 otx2b
Fish:	WT WT + MO1-gbx1 WT + MO2-gbx1
Knockdown Reagents:	MO1-gbx1 MO2-gbx1
Anatomical Terms:	forebrain neural keel midbrain neural keel presumptive rhombomere 3 presumptive rhombomere 5 segmental plate somite
Stage:	1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-gbx1 WT + MO2-gbx1
Knockdown Reagents:	MO1-gbx1 MO2-gbx1
Observed In:	hindbrain anterior region neural keel spinal cord neural keel
Stage:	1-4 somites

Phenotype Detail

Acknowledgments

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