ZFIN ID: ZDB-FIG-090113-30
Lin et al., 2009 - Distinct functions of Wnt/{beta}-catenin signaling in KV development and cardiac asymmetry. Development (Cambridge, England)   136(2):207-217 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 1 Wnt/β-catenin signaling regulates LR asymmetry. (A-E) APC is required for left-right patterning of zebrafish heart but not visceral organs. Wild-type sibling (A,C) and apc mutant (B,D) embryos were stained at 28 hpf (A,B) for cmlc2 and at 52 hpf (C,D) for trypsin. (E) Quantification of cardiac jogging and looping, and pancreas positioning. Shown are dorsal views with anterior to the top. (F-L) Activation and repression of Wnt/β-catenin signaling differentially affect cardiac and visceral organ laterality. (F-H) Representative images of cardiac looping as revealed by cmlc2 staining of 52 hpf embryos. Ventral views with anterior to the top. (I-K) Representative images of the positioning of visceral organs as revealed by foxa3 staining of 52 hpf embryos. Dorsal views with anterior to the top. l, liver; g, gut; p, pancreas. (L) Quantification of cardiac and visceral organ laterality. H, heart; G, gut and other visceral organs; n, total numbers of embryos scored.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myl7 TL standard conditions Prim-5 heart tube ISH
apchu745/hu745 standard conditions Prim-5 heart tube ISH
prss1 TL standard conditions Long-pec heart ISH
apchu745/hu745 standard conditions Long-pec heart ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TL + MO1-wnt3a standard conditions Long-pec embryonic heart tube left/right pattern formation disrupted, abnormal
Long-pec heart looping disrupted, abnormal
TL + MO1-wnt3a + MO1-wnt8a standard conditions Long-pec embryonic heart tube left/right pattern formation disrupted, abnormal
Long-pec heart looping disrupted, abnormal
TL + MO1-wnt8a standard conditions Long-pec embryonic heart tube left/right pattern formation disrupted, abnormal
Long-pec heart looping disrupted, abnormal
apchu745/hu745 standard conditions Prim-5 embryonic heart tube left/right pattern formation disrupted, abnormal
Prim-5 heart jogging disrupted, abnormal
Prim-5 presumptive atrium heart tube mislocalised radially, abnormal
Long-pec cardiac ventricle mislocalised radially, abnormal
Long-pec embryonic heart tube left/right pattern formation disrupted, abnormal
Long-pec heart looping disrupted, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development