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General Information
ZIRC
ZFIN ID: ZDB-FIG-090113-30
Lin et al., 2009 - Distinct functions of Wnt/{beta}-catenin signaling in KV development and cardiac asymmetry. Development (Cambridge, England)   136(2):207-217 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 1 Wnt/β-catenin signaling regulates LR asymmetry. (A-E) APC is required for left-right patterning of zebrafish heart but not visceral organs. Wild-type sibling (A,C) and apc mutant (B,D) embryos were stained at 28 hpf (A,B) for cmlc2 and at 52 hpf (C,D) for trypsin. (E) Quantification of cardiac jogging and looping, and pancreas positioning. Shown are dorsal views with anterior to the top. (F-L) Activation and repression of Wnt/β-catenin signaling differentially affect cardiac and visceral organ laterality. (F-H) Representative images of cardiac looping as revealed by cmlc2 staining of 52 hpf embryos. Ventral views with anterior to the top. (I-K) Representative images of the positioning of visceral organs as revealed by foxa3 staining of 52 hpf embryos. Dorsal views with anterior to the top. l, liver; g, gut; p, pancreas. (L) Quantification of cardiac and visceral organ laterality. H, heart; G, gut and other visceral organs; n, total numbers of embryos scored.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myl7 TL standard conditions Prim-5 heart tube ISH
apchu745/hu745 standard conditions Prim-5 heart tube ISH
prss1 TL standard conditions Long-pec heart ISH
apchu745/hu745 standard conditions Long-pec heart ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TL + MO1-wnt3a standard conditions Long-pec embryonic heart tube left/right pattern formation disrupted, abnormal
Long-pec heart looping disrupted, abnormal
TL + MO1-wnt3a + MO1-wnt8a standard conditions Long-pec embryonic heart tube left/right pattern formation disrupted, abnormal
Long-pec heart looping disrupted, abnormal
TL + MO1-wnt8a standard conditions Long-pec embryonic heart tube left/right pattern formation disrupted, abnormal
Long-pec heart looping disrupted, abnormal
apchu745/hu745 standard conditions Prim-5 embryonic heart tube left/right pattern formation disrupted, abnormal
apchu745/hu745 standard conditions Prim-5 heart jogging disrupted, abnormal
apchu745/hu745 standard conditions Prim-5 presumptive atrium heart tube mislocalised radially, abnormal
apchu745/hu745 standard conditions Long-pec cardiac ventricle mislocalised radially, abnormal
apchu745/hu745 standard conditions Long-pec embryonic heart tube left/right pattern formation disrupted, abnormal
apchu745/hu745 standard conditions Long-pec heart looping disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development