ZFIN ID: ZDB-FIG-090113-32
Lin et al., 2009 - Distinct functions of Wnt/{beta}-catenin signaling in KV development and cardiac asymmetry. Development (Cambridge, England)   136(2):207-217 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 26+ somites

Fig. 3 Activation of Wnt signaling ablates lefty2 expression in the heart without affecting the left-sided expression of spaw and pitx2. (A-P) Representative images of spaw expression in LPM (arrowheads in A-C,I-L), lefty2 expression in the cardiac field (arrows in E-G,I-K), and pitx2 expression in the posterior LPM (asterisks in M-O). Shown are dorsal views, with anterior to the top of 22- to 24-somite staged embryos. A tropomyosin probe was included in in situ hybridization to ensure embryos were at the proper stage, with the exception of pitx2 staining. (Q) Quantification of the percentages of asymmetric expression of spaw, lefty2 and pitx2. s, spaw; l, lefty2; p, pitx2. L, left side; A, absence; R, right side; B, bilateral; n, total numbers of embryos scored.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TL + MO1-wnt3a standard conditions 26+ somites embryonic heart tube left/right pattern formation disrupted, abnormal
TL + MO1-wnt8a standard conditions 26+ somites embryonic heart tube left/right pattern formation disrupted, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development