ZFIN ID: ZDB-FIG-090113-31
Lin et al., 2009 - Distinct functions of Wnt/{beta}-catenin signaling in KV development and cardiac asymmetry. Development (Cambridge, England)   136(2):207-217 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 10-13 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 10-13 somites

Fig. 2 Wnt/β-catenin signaling is essential for the LR patterning function of KV. (A-F) charon expression was suppressed in wnt3 (D), wnt8 (E), and wnt3/wnt8 double (F) morphants, but not in embryos overexpressing wnt3 (B) or wnt8 (C) RNA, as compared with control embryos (A). Shown are dorsal views of tail bud regions at the 12-somite stage. (G-N) Cilia development in KV was regulated by Wnt signaling, as shown by anti-acetylated tubulin antibody staining of 10-somite staged embryos. Compared with controls (G; n=15), shorter KV cilia were observed in wnt3 morphants (J; n=15), wnt8 morphants (K; n=12), and wnt3/wnt8 double morphants (L; n=15), but not in embryos injected with wnt3 RNA (H; n=12) or wnt8 RNA (I; n=12), as summarized in M. Cilia numbers were increased in embryos overexpressing wnt3 (H) or wnt8 (I) RNA but remained the same in wnt3 (J), wnt8 (K) and wnt3/wnt8 double (L) morphants, as summarized in N. Data shown are means±s.d. *P<0.001, Student's t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dand5 TL standard conditions 10-13 somites Kupffer's vesicle ISH
TL + MO1-wnt3a standard conditions 10-13 somites Kupffer's vesicle ISH
TL + MO1-wnt3a + MO1-wnt8a standard conditions 10-13 somites Kupffer's vesicle ISH
TL + MO1-wnt8a standard conditions 10-13 somites Kupffer's vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TL + MO1-wnt3a standard conditions 10-13 somites Kupffer's vesicle cilium decreased length, abnormal
TL + MO1-wnt3a + MO1-wnt8a standard conditions 10-13 somites Kupffer's vesicle cilium decreased length, abnormal
TL + MO1-wnt8a standard conditions 10-13 somites Kupffer's vesicle cilium decreased length, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development