ZFIN ID: ZDB-FIG-090106-12
Jiang et al., 2008 - Exdpf is a key regulator of exocrine pancreas development controlled by retinoic acid and ptf1a in zebrafish. PLoS Biology   6(11):e293 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Day 5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Protruding-mouth

Fig. 5 Reduced exdpf Causes Defects of Exocrine Cell Proliferation

(A) BrdU labeling result in 33 hpf embryos. Green: MP760:GFP, Red: BrdU staining. Blue: DAPI staining. Dorsal view, anterior to the left. Enlargement represents higher magnification of boxed area in each row. Arrows indicate non-proliferating cells; arrowheads indicate proliferating cells as evidenced by overlapping of red and green colors.

(B) BrdU labeling result in 3 dpf embryos. Green: elastase A:GFP, Red: BrdU staining. Lateral view, anterior to the left. Scale bar: 50 μm. Arrows indicate non-proliferating cells; arrowheads indicate proliferating cells as evidenced by overlapping of red and green colors.

(C) Quantitative graphs for BrdU incorporation rate in 33 hpf or 3 dpf embryos. The average number of GFP positive cells with BrdU incorporation was obtained by counting BrdU-labeled GFP positive cells from five embryos. Y axis: Mean ± SD.

(D) Semiquantitative RT-PCR examination of expression of cyclin D1 and cell cycle inhibitors p21, p27 and cyclin G1 in control (control), exdpf morphants (MO), and exdpf mRNA injected embryos (RNA) at 33 hpf, 2 dpf, 3 dpf, and 5 dpf. In each group, 30 embryos were used to extract total RNA for RT-PCR.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ccnd1 AB control Prim-15 to Day 5 whole organism RTPCR
AB + MO1-ppdpfa standard conditions Prim-15 to Day 5 whole organism RTPCR
ccng1 AB control Prim-15 to Day 5 whole organism RTPCR
AB + MO1-ppdpfa standard conditions Prim-15 to Day 5 whole organism RTPCR
cdkn1a AB control Prim-15 to Day 5 whole organism RTPCR
AB + MO1-ppdpfa standard conditions Prim-15 to Day 5 whole organism RTPCR
cdkn1bb AB control Prim-15 to Day 5 whole organism RTPCR
AB + MO1-ppdpfa standard conditions Prim-15 to Day 5 whole organism RTPCR
GFP Ab12-GFP gz2Tg chemical treatment: 5-bromo-2'-deoxyuridine Protruding-mouth exocrine cell IHC
Ab12-GFP Protruding-mouth exocrine pancreas IHC
Ab12-GFP gz2Tg + MO1-ppdpfa chemical treatment: 5-bromo-2'-deoxyuridine Protruding-mouth exocrine cell IHC
Ab12-GFP Protruding-mouth exocrine pancreas IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gz2Tg + MO1-ppdpfa chemical treatment: 5-bromo-2'-deoxyuridine Prim-15 cell fate specification disrupted, abnormal
Prim-15 cell population proliferation disrupted, abnormal
Prim-15 exocrine cell decreased amount, abnormal
Protruding-mouth cell population proliferation disrupted, abnormal
Protruding-mouth exocrine cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.