ZFIN ID: ZDB-FIG-080516-51
Minchin et al., 2008 - Sequential actions of Pax3 and Pax7 drive xanthophore development in zebrafish neural crest. Developmental Biology   317(2):508-522 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-5

Fig. S1 Phylogenetic analysis and expression of pax7b. A. Bayesian phylogenetic analysis of the Pax3/7 sub-family. The tree was rooted with D. melanogaster Gsb and Gsb-n. Confidence for nodes is indicated by Bayesian support values. Green and red boxes indicate Pax7 and Pax3 sub-groups, respectively. B. Multiple sequence alignment of Pax7b sequence with homologous Pax7 proteins. The green and blue bars above the sequences indicate the Paired box and homeodomain, respectively. Arrowheads denote exon boundaries. Red boxes denote identical residues. Grey boxes show similar residues. HS = Homo sapiens, MM = Mus musculus, GG = Gallus gallus, XL = Xenopus leavis, DR = Danio rerio. C-Q. In situ mRNA hybridisation analysis of pax7b (C-Q) alone or co-localised with either pax7 (L-N) or xdh (O-Q), Dorsal view flatmounts are anterior to top (C-H) and lateral view flatmounts are anterior to left and dorsal to top (I-K,L-Q). At 7s, pax7b is expressed in anterior rhombomeres (C,D arrow) and the anterior edge of the newly-formed somites (C,D, arrowheads). By 15s, pax7b expression is maintained in rhombomeric stripes and appears in midbrain and anterior spinal cord (D, arrows). Expression in somites (black boxes, magnified in E,F, white dots delineate a somite) is reduced in older somites (E), but abundant in the anterior region of young somites (F). At 25 hpf, pax7b is expressed in cranial NC (G,H arrows; MB = midbrain, r1 = rhombomere 1; white dashes CNS border). In trunk NC, pax7b is expressed primarily in nascent pre-migratory cells (arrows) in tail (I enlarged in K) and older migratory cells (arrowheads) in trunk (I enlarged in J). Pax7b co-localises with both pax7 (L-N) and xdh (O-Q) mRNA. Boxes (L,O) are magnified in M,N,P,Q to show regions of overlapping (arrows), and non-overlapping (arrowheads) mRNA. Xdh mRNA is mainly cytoplasmic (arrowheads, P,Q), whereas pax7b and pax7 mRNAs show prominent nuclear puncta (arrows, M,P).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aox5 WT standard conditions Prim-5 neural crest ISH
Prim-5 pigment cell ISH
pax7a WT standard conditions Prim-5 neural crest ISH
Prim-5 pigment cell ISH
pax7b WT standard conditions 5-9 somites hindbrain neural rod ISH
5-9 somites somite ISH
14-19 somites midbrain ISH
14-19 somites rhombomere ISH
14-19 somites somite ISH
14-19 somites spinal cord neural tube ISH
Prim-5 cranial neural crest cell ISH
Prim-5 neural crest cell ISH
Prim-5 pigment cell ISH
Prim-5 post-vent region ISH
Prim-5 trunk neural crest ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 317(2), Minchin, J.E., and Hughes, S.M., Sequential actions of Pax3 and Pax7 drive xanthophore development in zebrafish neural crest, 508-522, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.