ZFIN ID: ZDB-FIG-080516-23
Shin et al., 2008 - Multiple roles for Med12 in vertebrate endoderm development. Developmental Biology   317(2):467-479 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to Protruding-mouth

Fig. S1 shr is required for liver development. Wild-type and shrs435 mutant animals at 34 (A, A′), 52 (B, B′), and 72 (C, C′) hpf were analyzed for hhex (A, A′) and sepp1b (B, B′) expression and confocal projections of pan-Cadherin (red) and Islet (blue) expression (C, C′). (A, A′) prox1 expression is evident in the liver (white arrow) of wild-type embryos but barely detectable in the liver region of shrs435 mutants. (B, B′) selenoprotein P, plasma, 1b (sepp1b) expression is detected at low levels in shrs435 mutants (black arrow). (C, C′) shrs435 mutants exhibit defective differentiation of hepatocytes as assessed by Cadherin immunostaining. Note the multiple, unfused ventral pancreatic buds in mutants (white arrowheads). Yellow arrow points to the liver. A–B′ are dorsal views, anterior to the left; C, C′ are ventral views, anterior to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
prox1a WT standard conditions Long-pec liver ISH
med12s435/s435 standard conditions Long-pec liver ISH
selenop2 WT standard conditions Long-pec liver ISH
med12s435/s435 standard conditions Long-pec liver ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pan-Cadherin IHC WT standard conditions Protruding-mouth hepatocyte
IHC Protruding-mouth liver
IHC Protruding-mouth pancreas
IHC med12s435/s435 standard conditions Protruding-mouth hepatocyte
IHC Protruding-mouth liver
IHC Protruding-mouth pancreas
Ab2-isl IHC WT standard conditions Protruding-mouth pancreas
IHC med12s435/s435 standard conditions Protruding-mouth pancreas
Phenotype Details
Fish Conditions Stage Phenotype
med12s435/s435 standard conditions Prim-15 - Protruding-mouth endodermal cell fate specification disrupted, abnormal
med12s435/s435 standard conditions Prim-15 - Protruding-mouth liver decreased size, abnormal
med12s435/s435 standard conditions Prim-15 - Protruding-mouth liver morphology, abnormal
med12s435/s435 standard conditions Prim-15 - Protruding-mouth liver development disrupted, abnormal
med12s435/s435 standard conditions Prim-15 - Protruding-mouth pancreas morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 317(2), Shin, C.H., Chung, W.S., Hong, S.K., Ober, E.A., Verkade, H., Field, H.A., Huisken, J., and Stainier, D.Y., Multiple roles for Med12 in vertebrate endoderm development, 467-479, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.