ZFIN Figure: Shin et al., 2008, Fig. S2

ZFIN ID: ZDB-FIG-080516-24

ZFIN ID: ZDB-FIG-080516-24

Shin et al., 2008 - Multiple roles for Med12 in vertebrate endoderm development. Developmental Biology 317(2):467-479 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	DsRed GFP
Fish:	gz2Tg; gz4Tg; m1018Tg med12^s435/s435; gz2Tg; gz4Tg; m1018Tg
Anatomical Terms:	endocrine pancreas exocrine pancreas liver
Stage:	Day 4

PHENOTYPE:

Fish:	med12^s435/s435; gz2Tg; gz4Tg; m1018Tg
Observed In:	endodermal cell fate specification exocrine pancreas exocrine pancreas development liver
Stage:	Day 4

Fig. S2 shr is required for ventral pancreas development. Confocal projections of wild-type and shr^s435 mutant larvae in the Tg(fabp1a:dsRed, elastase:GFP; insulin:dsRed) line at 96 hpf. (A, A′) shr^s435 mutant larvae show multiple Tg(elastase: GFP)-positive ventral pancreatic buds (white arrowheads), and defective elongation of the ventral pancreatic tail (white arrow). Despite defective hepatic differentiation, a population of liver cells expresses DsRed in the mutant larvae. All images are ventral views, anterior to the top.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
DsRed	gz2Tg; gz4Tg; m1018Tg	standard conditions	Day 4	endocrine pancreas	IFL
			Day 4	liver	IFL
	med12^s435/s435; gz2Tg; gz4Tg; m1018Tg	standard conditions	Day 4	endocrine pancreas	IFL
			Day 4	liver	IFL
GFP	gz2Tg; gz4Tg; m1018Tg	standard conditions	Day 4	exocrine pancreas	IFL
	med12^s435/s435; gz2Tg; gz4Tg; m1018Tg	standard conditions	Day 4	exocrine pancreas	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
med12^s435/s435; gz2Tg; gz4Tg; m1018Tg	standard conditions	Day 4	endodermal cell fate specification disrupted, abnormal
		Day 4	exocrine pancreas development disrupted, abnormal
		Day 4	exocrine pancreas increased amount, abnormal
		Day 4	exocrine pancreas morphology, abnormal
		Day 4	liver decreased size, abnormal
		Day 4	liver morphology, abnormal

Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from Developmental Biology, 317(2), Shin, C.H., Chung, W.S., Hong, S.K., Ober, E.A., Verkade, H., Field, H.A., Huisken, J., and Stainier, D.Y., Multiple roles for Med12 in vertebrate endoderm development, 467-479, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.

Acknowledgments:

ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 317(2), Shin, C.H., Chung, W.S., Hong, S.K., Ober, E.A., Verkade, H., Field, H.A., Huisken, J., and Stainier, D.Y., Multiple roles for Med12 in vertebrate endoderm development, 467-479, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.