ZFIN ID: ZDB-FIG-080516-22
Shin et al., 2008 - Multiple roles for Med12 in vertebrate endoderm development. Developmental Biology   317(2):467-479 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 7 Med12 functions with Cas/Sox32 and Foxa2 to regulate endodermal organ development. (A–C) Embryos from med12s432 heterozygote incrosses injected with cas mRNA were harvested at 90% epiboly and analyzed for sox17 expression. med12s432 homozygous mutant embryos overexpressing cas mRNA showed decreased induction of sox17 expression (C) compared to med12 heterozygous (B) and wild-type (A) embryos. (D–H′) Embryos from wild-type crosses and med12s432 heterozygote incrosses injected with foxa2 MO were harvested at 48 hpf and analyzed for foxa3 expression. Wild-type foxa3 expression reveals structures such as the liver (black arrow), pancreas (black arrowhead), and swim bladder (white arrowhead) (D). med12s432 mutants show an ambiguous ductal morphology (black bracket) and defective development of endodermal organs (E). foxa2 MO-injected embryos showed slight perturbation of organ development (F), while med12s432 heterozygous embryos injected with foxa2 MO showed a more severe malformation of organs (G), and med12s432 homozygous mutant embryos injected with foxa2 MO showed an almost complete absence of accessory organs, exhibiting a solid endodermal rod (H, H′). (I–K) Confocal projections of larvae from med12s432 heterozygote outcrosses in the Tg (fabp1a:dsRed, elastase:GFP; insulin:dsRed) heterozygote line injected with foxa2 MO (J and K) and analyzed at 96 hpf. While foxa2 MO-injected larvae showed slight perturbation of liver and exocrine pancreas (J), med12s432 heterozygous larvae with foxa2 MO injection showed a more severe malformation of these organs (K). Images A–C are dorsal views, anterior to the top; D–H′ are dorsal views, anterior to the left; I–K are ventral views, anterior to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed gz2Tg; gz4Tg; m1018Tg standard conditions Day 4 endocrine pancreas IFL
Day 4 liver IFL
gz2Tg; gz4Tg; m1018Tg + MO1-foxa2 standard conditions Day 4 endocrine pancreas IFL
Day 4 liver IFL
med12s432/+; gz2Tg; gz4Tg; m1018Tg + MO1-foxa2 standard conditions Day 4 endocrine pancreas IFL
Day 4 liver IFL
foxa3 WT standard conditions Long-pec gut ISH
Long-pec liver ISH
Long-pec pancreas ISH
Long-pec swim bladder bud ISH
WT + MO1-foxa2 standard conditions Long-pec gut ISH
Long-pec liver ISH
Long-pec pancreas ISH
med12s432/+ + MO1-foxa2 standard conditions Long-pec gut ISH
Long-pec liver ISH
Long-pec pancreas ISH
med12s432/s432 standard conditions Long-pec gut ISH
Long-pec liver ISH
Long-pec pancreas ISH
med12s432/s432 + MO1-foxa2 standard conditions Long-pec gut ISH
Long-pec Not Detected liver ISH
Long-pec Not Detected pancreas ISH
GFP gz2Tg; gz4Tg; m1018Tg standard conditions Day 4 pancreas IFL
gz2Tg; gz4Tg; m1018Tg + MO1-foxa2 standard conditions Day 4 pancreas IFL
med12s432/+; gz2Tg; gz4Tg; m1018Tg + MO1-foxa2 standard conditions Day 4 pancreas IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-foxa2 standard conditions Long-pec pancreas decreased size, abnormal
Long-pec pancreas development disrupted, abnormal
gz2Tg; gz4Tg; m1018Tg + MO1-foxa2 standard conditions Day 4 exocrine pancreas decreased size, abnormal
Day 4 liver decreased size, abnormal
Day 4 liver development disrupted, abnormal
Day 4 pancreas development disrupted, abnormal
med12s432/+ + MO1-foxa2 standard conditions Long-pec liver decreased size, abnormal
Long-pec liver development disrupted, abnormal
Long-pec pancreas decreased size, abnormal
Long-pec pancreas development disrupted, abnormal
med12s432/+; gz2Tg; gz4Tg; m1018Tg + MO1-foxa2 standard conditions Day 4 exocrine pancreas decreased size, abnormal
Day 4 liver decreased size, abnormal
Day 4 liver development disrupted, abnormal
Day 4 pancreas development disrupted, abnormal
med12s432/s432 standard conditions Long-pec hepatic duct morphology, abnormal
Long-pec liver development disrupted, abnormal
Long-pec pancreas development disrupted, abnormal
Long-pec pancreatic duct morphology, abnormal
med12s432/s432 + MO1-foxa2 standard conditions Long-pec liver aplastic, abnormal
Long-pec liver development disrupted, abnormal
Long-pec pancreas aplastic, abnormal
Long-pec pancreas development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 317(2), Shin, C.H., Chung, W.S., Hong, S.K., Ober, E.A., Verkade, H., Field, H.A., Huisken, J., and Stainier, D.Y., Multiple roles for Med12 in vertebrate endoderm development, 467-479, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.