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ZFIN ID: ZDB-FIG-080422-7
Chung et al., 2008 - Intra-endodermal interactions are required for pancreatic beta cell induction. Developmental Cell   14(4):582-593 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Pec-fin
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Pec-fin

Fig. 3 Shh Compensates for the Loss of Axial Mesoderm in the Formation of Pancreatic Endocrine Cells

(A–C) Ventral confocal images of Tg(ins:GFP) (green) and Tg(sox17:DsRed)s903 (red) expression, comparing wild-type (A), flh mutants (B), and shh-overexpressing flh mutants (C) at 18 hpf. (C) At 18 hpf, Tg(ins:GFP)-expressing cells were rescued in shh-overexpressing flh mutants and scattered within the endodermal sheet (red). Most endodermal cells failed to converge following shh overexpression, leading to the presence of gaps (asterisks) within the endodermal sheet.

(D–I) Lateral (D–F) and dorsal (G–I) views of Tg(ins:GFP) embryos comparing wild-type (D and G), flh mutants (E and H), and shh-overexpressing flh mutants (F and I) at 24 hpf. (D and G) Wild-type embryos show one cluster of Tg(ins:GFP)-expressing cells (arrow). (E and H) Tg(ins:GFP) expression is completely missing in flh mutants. (F and I) shh-overexpressing flh mutants show rescue of Tg(ins:GFP) expression (arrow).

(J–O) Confocal projections of pancreatic endocrine cells comparing wild-type (J and M), flh mutants (K and N), and shh-overexpressing flh mutants (L and O) at 48 (J–L) and 60 (M–O) hpf. Tg(ins:GFP) embryos were stained for GFP (green), Somatostatin (blue), and Glucagon (red). flh mutants show dramatically reduced pancreatic endocrine cells at 48 (K) and 60 (N) hpf, compared to wild-type (J and M). Tg(ins:GFP) and Somatostatin expression were rescued at 48 hpf (L), and Glucagon expression was also detectable at 60 hpf (O) in shh-overexpressing flh mutants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed noton1/n1; s903Tg; zf5Tg standard conditions 14-19 somites endoderm IFL
s903Tg; zf5Tg standard conditions 14-19 somites endoderm IFL
GFP noton1/n1; s903Tg; zf5Tg standard conditions 14-19 somites Not Detected whole organism IFL
noton1/n1; zf5Tg standard conditions Prim-5 Not Detected whole organism IFL
Long-pec endocrine cell IFL
Long-pec pancreas IFL
Pec-fin endocrine cell IFL
Pec-fin pancreas IFL
s903Tg; zf5Tg standard conditions 14-19 somites endoderm IFL
zf5Tg standard conditions Prim-5 endoderm IFL
Prim-5 pancreas primordium IFL
Long-pec endocrine pancreas IFL
Pec-fin endocrine pancreas IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
noton1/n1; zf5Tg standard conditions Long-pec pancreas development disrupted, abnormal
Long-pec pancreas endocrine cell decreased amount, abnormal
Pec-fin pancreas development disrupted, abnormal
Pec-fin pancreas endocrine cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 14(4), Chung, W.S., and Stainier, D.Y., Intra-endodermal interactions are required for pancreatic beta cell induction, 582-593, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell