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ZFIN ID: ZDB-FIG-080422-17
Chung et al., 2008 - Intra-endodermal interactions are required for pancreatic beta cell induction. Developmental Cell   14(4):582-593 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: 14-19 somites

Fig. S6 Direct Endodermal Cell-Cell Contacts May Not Be Essential for Tg(ins:GFP) Expression
(A-B) Ventral confocal images of Tg(ins:GFP) (green), Tg(sox17:DsRed)s903 (red) and β- catenin (blue) expression, comparing wildtype (A) and cas MO (0.1∼0.4 ng) injected (B-D) embryos at 18 hpf (mid-trunk region). (A) In wildtype embryos, Tg(sox17:DsRed)s903 expressing endodermal cells are condensed near the midline and Tg(ins:GFP) expressing cell are located medially (arrowheads). (B) A majority (n = 53/67) of cas MO injected embryos did not exhibit Tg(ins:GFP) expression and showed a much reduced number of Tg(sox17:DsRed)s903 expressing cells. These endodermal cells (arrows) were located away from the midline (asterisks). (C-D) In the other 14 embryos, a few endodermal cells (arrows) had migrated close to the midline (asterisks) and were expressing the ins:GFP transgene (arrowheads). (D) Interestingly, in 4 such embryos, a single, isolated Tg(ins:GFP) expressing cell was observed (double arrow). Examining a set of 14-somite stage embryos led to similar observations (Table S4). It is interesting to note that in these experimental embryos, Tg(ins:GFP) expression was mostly observed close to the midline, possibly revealing the importance of medio-lateral information in the dorsal pancreatic β- cell induction process.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed s903Tg; zf5Tg standard conditions 14-19 somites endodermal cell IFL
s903Tg; zf5Tg + MO3-sox32 standard conditions 14-19 somites endodermal cell IFL
GFP s903Tg; zf5Tg standard conditions 14-19 somites endodermal cell IFL
s903Tg; zf5Tg + MO3-sox32 standard conditions 14-19 somites endodermal cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 14(4), Chung, W.S., and Stainier, D.Y., Intra-endodermal interactions are required for pancreatic beta cell induction, 582-593, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell