ZFIN ID: ZDB-FIG-080403-25
Koudijs et al., 2008 - Genetic analysis of two zebrafish patched homologues identifies novel roles for the hedgehog signaling pathway. BMC Developmental Biology   8:15 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-15

Fig. 2 Phenotypic consequences of zebrafish ptc1 mutants. Homozygous ptc1hu1602 mutants show a subtle somite phenotype at 32 hpf, where the average angle of the somite becomes more obtuse (A,B). At 72 hpf, ptc1hu1602 mutants exhibit an eye phenotype where the pigmented epithelium is extended into the diencephalon. The similar phenotype described for the blw mutant is weaker compared to the ptc1hu1602 mutant (C-E). The expression level of ptc1, a general readout for Hh activity, shows a mild increase in the blw mutant compared to wild type (F-H). The ptc1hu1602 mutant shows a severely increased level of ptc1, where wild types, heterozygotes and mutants can be distinguished based on ptc1 levels (I-K). Additional to the difference in the strength of the eye phenotype, the activation of the pathway is significantly higher in ptc1hu1602 mutants compared to blw mutants. An increased expression level of gli1 confirms an activation of the Hh pathway in the ptc1hu1602 mutant (L-N).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gli1 WT standard conditions 14-19 somites brain ISH
14-19 somites somite ISH
ptch2hu1602/+ standard conditions 14-19 somites brain ISH
14-19 somites somite ISH
ptch2hu1602/hu1602 standard conditions 14-19 somites brain ISH
14-19 somites somite ISH
ptch2 WT standard conditions 14-19 somites brain ISH
14-19 somites notochord ISH
ptch2hu1602/+ standard conditions 14-19 somites brain ISH
14-19 somites notochord ISH
ptch2hu1602/hu1602 standard conditions 14-19 somites brain ISH
14-19 somites notochord ISH
ptch2tc294z/+ standard conditions 14-19 somites brain ISH
14-19 somites notochord ISH
ptch2tc294z/tc294z standard conditions 14-19 somites brain ISH
14-19 somites notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ptch2hu1602/hu1602 standard conditions Prim-15 somite obtuse angle to somite, abnormal
Prim-15 somite orientation somite, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.