FIGURE

Fig. 2

ID

ZDB-FIG-070129-19

Publication

Jeong et al., 2006 - Neurogenin1 is a determinant of zebrafish basal forebrain dopaminergic neurons and is regulated by the conserved zinc finger protein Tof/Fezl

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Fig. 2

ngn1 is required for DA neuron development in the basal forebrain. All images are lateral views of anterior brain regions except I–L. Anterior is to the left, and dorsal is up. (A–L) Immunostaining with TH antibody (A, B, G, and L), in situ with TH (E and F), or DAT antisense probe (C and D), or immunostaining with 5HT antibody (M and N), shows that DA neurons in the ventral forebrain is completely absent at 32 hpf, largely absent at 48 hpf, and remain significantly defective at 72 hpf in ngn1-morphants, whereas TH⁺ DA neurons appear normal in the retina, TH⁺ gut sympathetic neurons are normal in the trunk region of ngn1 morphants, and 5HT neurons are not affected. (Insets) Magnified views of basal forebrain 5HT neurons. DA, dopaminergic neurons; DAT, dopamine transporter; di, diencephalon; epi, epiphysis; hb, hindbrain; LC, locus coeruleus; mhb, midhindbrain boundary; t, telencephalon. (Scale bar, 32 μm.)

Expression Data

Genes:	slc6a3 th
Fish:	WT WT + MO3-neurog1
Knockdown Reagent:	MO3-neurog1
Anatomical Terms:	dopaminergic neuron forebrain gut locus coeruleus retina
Stage Range:	Prim-15 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-neurog1
Knockdown Reagent:	MO3-neurog1
Observed In:	forebrain dopaminergic neuron
Stage:	Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA