ZFIN ID: ZDB-FIG-060620-3
Villablanca et al., 2006 - Control of cell migration in the zebrafish lateral line: Implication of the gene "Tumour-Associated Calcium Signal Transducer," tacstd. Developmental dynamics : an official publication of the American Association of Anatomists   235(6):1578-1588 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Adult

Fig. 3 Developmental expression of tacstd. A: RT-PCR detection of tacstd message at 0, 6, 24, 48 haf, 6 daf, and adult (Ad) stages. Amplification of Wnt5a message is used as a positive control. The adult mRNA sample was contaminated with genomic DNA as amplification of genomic sequence produces a larger product (labelled as "genomic"). B-D: Whole mount in situ hybridization using a tacstd riboprobe. Embryos were processed for hybridization at 23 (B), 28 (C), and 48 haf (D). E-G: Neuromasts of in situ hybridized embryos. Probes used were tacstd (E), ath1 (F), or both probes (G). H,I: tacstd expression in the anterior lateral line (H) or posterior lateral line (I) primordia. Arrows indicate direction of migration. Dotted circles demarcate cells showing a rosette pattern, which is coincident with strong expression of tacstd. Expression to the left of the primordium in I is a recently deposited proneuromast. ALL, anterior lateral line; L1-5, posterior lateral line neuromasts; O1,2, otic neuromasts; OP, olfactory placode; OV, otic vesicle; PLL, posterior lateral line; prim, primordium.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
epcam WT standard conditions 1-cell to Adult whole organism RTPCR
26+ somites anterior lateral line placode ISH
26+ somites olfactory placode ISH
26+ somites otic vesicle ISH
26+ somites posterior lateral line primordium ISH
Prim-5 anterior lateral line system ISH
Prim-5 neuromast ISH
Prim-5 olfactory placode ISH
Prim-5 otic vesicle ISH
Prim-5 posterior lateral line ganglion ISH
Prim-5 posterior lateral line primordium ISH
Prim-25 anterior lateral line primordium ISH
Prim-25 anterior lateral line system ISH
Prim-25 neuromast ISH
Prim-25 posterior lateral line primordium ISH
wnt5a WT standard conditions 1-cell to Adult whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.