ZFIN ID: ZDB-FIG-051221-2
Chen et al., 2005 - Loss of function of def selectively up-regulates {Delta}113p53 expression to arrest expansion growth of digestive organs in zebrafish. Genes & Development   19(23):2900-2911 Full text @ Genes & Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec

Fig. S3 def-MO caused hypoplastic digestive organs as that observed in the defhi429 mutant. (A,B) def-MO (5′-ATGAATATAATGACTTACCAAGCGC-3′) was designed to target the splice junction between exon 2 and intron 2. RT-PCR analysis showed that def-MO created an aberrant frame-shift transcript (A) due to 40 bp deletion in the exon 2 (B). (MO) def-MO; (SC) standard control morpholinos; (C) wild type contol. (C) def-MO morphants were examined with intestinal marker ifabp, liver marker lfabp and pancreas marker trypsin, respectively. 19/27 (70.4%) morphants showed hypoplastic intestine tube (ifabp); 35/47 (74.5%) for liver (lfabp), and 50/56 (89.3%) for pancreas (trypsin).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fabp2 WT standard conditions Long-pec gut ISH
WT + MO1-utp25 standard conditions Long-pec gut ISH
fabp10a WT standard conditions Long-pec liver ISH
WT + MO1-utp25 standard conditions Long-pec liver ISH
prss1 WT standard conditions Long-pec pancreas ISH
WT + MO1-utp25 standard conditions Long-pec pancreas ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Full text @ Genes & Dev.