|ZFIN ID: ZDB-FIG-051221-2|
Fig. S3 def-MO caused hypoplastic digestive organs as that observed in the defhi429 mutant. (A,B) def-MO (5′-ATGAATATAATGACTTACCAAGCGC-3′) was designed to target the splice junction between exon 2 and intron 2. RT-PCR analysis showed that def-MO created an aberrant frame-shift transcript (A) due to 40 bp deletion in the exon 2 (B). (MO) def-MO; (SC) standard control morpholinos; (C) wild type contol. (C) def-MO morphants were examined with intestinal marker ifabp, liver marker lfabp and pancreas marker trypsin, respectively. 19/27 (70.4%) morphants showed hypoplastic intestine tube (ifabp); 35/47 (74.5%) for liver (lfabp), and 50/56 (89.3%) for pancreas (trypsin).
|WT + MO1-utp25||standard conditions||Long-pec||gut||ISH|
|WT + MO1-utp25||standard conditions||Long-pec||liver||ISH|
|WT + MO1-utp25||standard conditions||Long-pec||pancreas||ISH|
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