ZFIN ID: ZDB-FIG-051220-27
Chen et al., 2005 - Loss of function of def selectively up-regulates {Delta}113p53 expression to arrest expansion growth of digestive organs in zebrafish. Genes & Development   19(23):2900-2911 Full text @ Genes & Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5

Fig. 5 Loss of function of def selectively up-regulates the expression of {Delta}113p53 in the defhi429 mutant. (A) 5'-RACE reaction identified two p53 isoforms. The longer isoform corresponds to the wild-type p53 (p53) and was predominant in the wild-type embryos. The short isoform ({Delta}113p53) was drastically increased in the defhi429 mutant. (B) The short isoform contains 155 bp derived from intron 4 (letters in lowercase) immediately adjacent to exon 5 and encodes for an N-truncated p53 protein initiated at codon 113 of the wild-type p53. (C) RNA gel blot hybridization using probes for detecting both p53 and {Delta}113p53 isoforms (P1 probe, top panel), p53 only (P2 probe, second panel), and {Delta}113p53 only (P3 probe, third panel) showed that {Delta}113p53 but not p53 was selectively increased in the def-MO morphants (def-MO) and defhi429 mutant (mu), respectively, when compared with the standard control MO morphants (SC-MO) and the wild-type control (wt). (Bottom panel) 28S rRNA was used as the loading control. (D) Whole-mount in situ hybridization using the P1 probe showed that {Delta}113p53 expression in the defhi429 mutant (mu, right panels) was increased at 3 dpf, 4 dpf, and 5 dpf, and this increase was specifically limited to within digestive organs when compared with the expression of p53 in the wild type (left panels). (in) Intestine; (lv) liver; (p) pancreas; (sw) swimbladder. (E,F) At 4 dpf, sectioning in situ hybridization further confirmed that p53 expression was increased in the intestine (in), liver (data not shown), and exocrine pancreas (ex) but not in the endocrine pancreas (en) in the defhi429 mutant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tp53 WT standard conditions Protruding-mouth to Day 5 exocrine pancreas ISH
Protruding-mouth to Day 5 gut ISH
Protruding-mouth to Day 5 liver ISH
Protruding-mouth to Day 5 pharynx ISH
Day 5 unspecified NB
WT + MO1-utp25 standard conditions Protruding-mouth to Day 4 unspecified NB
utp25hi429Tg standard conditions Protruding-mouth to Day 5 exocrine pancreas ISH
Protruding-mouth to Day 5 gut ISH
Protruding-mouth to Day 5 liver ISH
Protruding-mouth to Day 5 pharynx ISH
Day 5 unspecified NB
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Genes & Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.
In addition, ZFIN would like to thank the authors for kindly granting us permission to reproduce their figures.
Full text @ Genes & Dev.