ZFIN ID: ZDB-FIG-050307-6
Amoyel et al., 2005 - Wnt1 regulates neurogenesis and mediates lateral inhibition of boundary cell specification in the zebrafish hindbrain. Development (Cambridge, England)   132(4):775-785 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5

Fig. 6 Repression of boundary markers by proneural genes and deltaA. Expression at 24 hours of rfng (A-H) and foxb1.2 (fox, I-L) in uninjected embryos (A,I) and embryos injected with morpholinos to block proneural gene or deltaA function, as indicated (B-H,J-L). The probe is indicated at the top right of each panel. Expansion of rfng and foxb1.2 boundary expression occurs in proneural gene knockdowns (121/145 embryos), and following deltaA knockdown (24/24 embryos). Arrowheads in I indicate boundary expression of foxb1.2, which is broader in MO embryos (brackets, J-L). Different strengths of phenotype are observed following proneural gene knockdown, asha knockdown being the strongest (B) and ngn1 the weakest (E). Ectopic expression is not seen in r4, even in a knockdown of asha, ashb plus ngn1 (G). Scale bar: 100 ┬Ám

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxb1a WT standard conditions Prim-5 hindbrain ISH
WT + MO1-ascl1b standard conditions Prim-5 hindbrain ISH
WT + MO1-ascl1b + MO3-ascl1a standard conditions Prim-5 hindbrain ISH
WT + MO1-dla standard conditions Prim-5 hindbrain ISH
rfng WT standard conditions Prim-5 hindbrain ISH
WT + MO1-ascl1b standard conditions Prim-5 hindbrain ISH
WT + MO1-ascl1b + MO3-ascl1a standard conditions Prim-5 hindbrain ISH
WT + MO1-ascl1b + MO3-ascl1a + MO5-neurog1 standard conditions Prim-5 hindbrain ISH
WT + MO1-dla standard conditions Prim-5 hindbrain ISH
WT + MO3-ascl1a standard conditions Prim-5 hindbrain ISH
WT + MO5-neurog1 standard conditions Prim-5 hindbrain ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development