FIGURE SUMMARY
Title

A dataset of transcriptomic effects of camptothecin treatment on early zebrafish embryos

Authors
Prykhozhij, S.V., Ban, K., Brown, Z.L., Kobar, K., Wajnberg, G., Fuller, C., Chacko, S., Lacroix, J., Crapoulet, N., Midgen, C., Shlien, A., Malkin, D., Berman, J.N.
Source
Full text @ Data Brief
Fig. 1

Global overview of differential gene expression after camptothecin (CPT) treatment of zebrafish embryos. (A) Heatmap plot of all DEGs after CPT treatment. Standard hierarchical clustering was performed of the variance-stabilized values which were scaled row-wise for visualization. Treatment and genotype factors are indicated in the heatmap. Numbers of up- and down-regulated genes are indicated in the side panel of the heatmap. (B) MA plot of all genes with non-DEG genes labeled in grey, up-regulated genes in red and down-regulated in blue. The names of top 80 DEGs are indicated directly on the plot. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Fig. 2

GO BP and KEGG Pathways terms enriched among the DEGs due to CPT treatment. Barplots of the Negative Log10 transformation of P-values for Gene Ontology Biological Process (A) and KEGG pathway (B) terms. (A) All of the GO BP terms associated with up-regulated genes (shown in red) are plotted as well as top 25 GO BP terms linked to down-regulated genes (light-blue). Separate plots are due to different scales of Negative Log10 of P-value. (B) KEGG pathway term plot. Direction of gene regulation is indicated by the legend on both sets of plots. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Fig. 3

Heatmap of expression values for interferon-regulated genes (IRG) induced or repressed by DNA damage due to camptothecin treatment. Heatmap of normalized variance-stabilized gene expression values for known IRGs. To simplify presentation, averages of 3 biological replicates belonging to each treatment group are shown in the heatmap. Both up and down-regulated IRGs sorted in descending order by fold change are shown in the heatmap. The values were scaled row-wise. Treatment and genotype assignments are indicated by color bars above the heatmap, and the legend is provided at the top right.
Fig. 4

Heatmap of expression values for p53 target genes induced or repressed by DNA damage due to camptothecin treatment. Heatmap of normalized variance-stabilized gene expression values for known zebrafish orthologs of known human p53 target genes. Averages of 3 biological replicates belonging to each treatment group are shown in the heatmap. Both up and down-regulated p53 targets sorted in descending order by fold change are shown in the heatmap. The values were scaled row-wise. Treatment and genotype assignments are indicated by color bars above the heatmap, and the legend is provided on the bottom right.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Data Brief
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