Effects of retene exposure on zebrafish development at 24 and 120 hpf in wild type and AHR2 mutant fish. (A) heatmap of endpoint incidence. Depth of color indicates percent incidence (x-axis) at given concentration (y-axis); (B) concentration-response curves for percent incidence of teratogenicity at 24 or 120 hpf; (C) 120 hpf larvae resulting from exposure to 50 µM retene beginning at six hpf, with and without functional AHR2.

Concentration-response curve for percent incidence of any teratogenic effect observed at 24 or 120 hpf. Exposure concentrations for toxicity screening were matched to those for transcriptomic assessment.

(A) Number of differentially expressed genes and their corresponding Log2 fold change values at each retene concentration (adj. p < 0.05). Cyp1a is highlighted red. (B) Venn diagram of DEGs at concentrations with and without teratogenicity at 120 hpf. Overlapping region represents genes which were differentially expressed in at least one non-teratogenic and one teratogenic concentration.

Heatmap of transcriptomic response at each retene concentration for genes common to both non-teratogenic and teratogenic concentrations. Average Log2FC values for each gene at each treatment are displayed with yellow indicating higher expression and blue indicating lower expression compared to DMSO vehicle control. Genes are grouped by ward clustering with gene clusters on the left.

Gene Ontology and Reactome pathway enrichment for DEGs unique to non-teratogenic concentrations, DEGs overlapping at least one non-teratogenic and one teratogenic concentration, and DEGs unique to teratogenic concentrations.

Concentration-response curves for black and green cluster genes which were differentially expressed in at four concentrations. Gene symbol and BMC are displayed in each panel.

Gene co-expression network inferred from all retene concentrations. The largest 13 modules are differentiated by color. The inset box highlights Module seven which contained the greatest number of high responding genes. Black and green cluster genes which showed the strongest change in expression in response to increasing retene concentration are denoted.