Fig. 1

Fig. 1. Configuration of Airy-like LSFM.

Fig. 2

Fig. 2. Simulated data for light-sheet illumination with Airy and Airy-like beams. (A-B) Propagation profiles of Airy light-sheet and Airy-like light-sheet along x axis. (C-D) MTF (x, fz) with threshold line at 5%. (E-H) Simulated, recorded, and deconvolved images of the University logo. (I-L) Two-dimensional MTF of Airy beam light-sheet at different planes beyond the waist. (M-P) Two-dimensional MTF of Airy-like beam light-sheet at different planes beyond the waist. (Q-T) MTF for the two light-sheets using single photon excitation at different planes beyond the waist. The spatial frequency, is normalized to 2NA/λ, where λ and NA are the illumination wavelength and the numerical aperture of illumination objective respectively.

Fig. 3

Fig. 3. Calibration data with standard fluorescent microspheres for Airy (NA= 0.27), Airy-like (NA= 0.27) and Airy (NA= 0.19) light-sheet illuminations. (A, B, C) Deconvolved images of fluorescent microspheres from maximum intensity projections of recorded data. (D) Peak intensity as a function of propagation distance (FOV) for the microspheres illuminated with the conventional Airy (NA= 0.27) light-sheet (pink), the Airy-like (NA= 0.27) light-sheet (blue) and Airy (NA= 0.19) light-sheet (black). To evaluate the illumination uniformity, normal distributions have been fitted to the intensities. The FOV based on normalized fluorescence intensity drop to 1 / e for Airy light-sheet and Airy like light-sheet (E) Full-width at half maximum (FWHM) in z axial of the microsphere images.

Fig. 4

Fig. 4. Simulated data for light-sheet illumination with two-photon Airy-like and two-photon planner Airy-like beam. (A, B) Propagation profiles of 2-PE Airy-like light-sheet and 2-PE Airy light-sheet along x axis. (C, D) MTF (x, fz) with threshold line at 5%. (E-H) Simulated, recorded, and deconvolved images of the University logo. (I-L) MTF for the two light-sheets using 2-PE at different planes beyond the waist. The spatial frequency, is normalized to 2NA/λ, where λ and NA are the illumination wavelength and the numerical aperture of illumination objective respectively.

Fig. 5

Fig. 5. Comparison of planar Airy light-sheet and planar Airy-like light-sheet using 2-PE with standard fluorescent microspheres. (A, B, C) Maximum intensity projections of recorded data of fluorescent microspheres. (D) Peak intensity as a function of propagation distance (FOV) for the microspheres illuminated with the 2-PE Airy-like light-sheet (black) and the 2-PE planar Airy-like light-sheet (red). (E) Full-width at half maximum (FWHM) in z axial of the microsphere images. (F, G) Maximum intensity projection of microspheres near the edge of FOV for Airy-like light-sheet in (E) and planar Airy-like light-sheet in (A, B). (H) The intensity profile of the microspheres in panels (E) and (F).

Fig. 6

Fig. 6. Real-time monitoring of ICH in zebrafish model. (A) The distribution of cerebral blood vessels (green) and red blood cells (red) in zebrafish larva. (B-E) partial 3D diagrams of blood vessel (PrA), corresponding to the position of the yellow dashed box as shown in panel(A). (F-M) Dynamic change of blood vessels from 50.5 to 54hpf (see Visualization 2, Visualization 3, Visualization 4, and Visualization 5).

Fig. 7

Fig. 7. Analysis of changes in fluorescence intensity of blood vessels (green) and red blood cells (red) within bleeding area. (A) Analysis of changes in fluorescence intensity of blood vessels in the yellow dashed box, with panels on the left and that of red blood cells on the right. (B) Fluorescence intensity ratio of blood vessels and red blood cells in the three bleeding areas at different time points. The value of the fluorescence ratio is derived from the ratio of the total fluorescence of the sub-area to the total fluorescence of a certain area, and data are shown as mean ± standard deviation.

Acknowledgments

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