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Fig. 3
Fig. 3. Calibration data with standard fluorescent microspheres for Airy (NA= 0.27), Airy-like (NA= 0.27) and Airy (NA= 0.19) light-sheet illuminations. (A, B, C) Deconvolved images of fluorescent microspheres from maximum intensity projections of recorded data. (D) Peak intensity as a function of propagation distance (FOV) for the microspheres illuminated with the conventional Airy (NA= 0.27) light-sheet (pink), the Airy-like (NA= 0.27) light-sheet (blue) and Airy (NA= 0.19) light-sheet (black). To evaluate the illumination uniformity, normal distributions have been fitted to the intensities. The FOV based on normalized fluorescence intensity drop to 1 / e for Airy light-sheet and Airy like light-sheet (E) Full-width at half maximum (FWHM) in z axial of the microsphere images.
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