PUBLICATION
Airy-like beam-based light-sheet microscopy with improved FOV for zebrafish intracerebral hemorrhage
- Authors
- Liu, P., Chen, H., Pang, M., Liu, X., Wang, J., Zhang, X.D., Ming, D.
- ID
- ZDB-PUB-220428-2
- Date
- 2022
- Source
- Optics express 30: 14709-14722 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cerebral Hemorrhage/diagnostic imaging
- Larva
- Microscopy*/methods
- Photons
- Zebrafish*
- PubMed
- 35473209 Full text @ Opt. Express
Citation
Liu, P., Chen, H., Pang, M., Liu, X., Wang, J., Zhang, X.D., Ming, D. (2022) Airy-like beam-based light-sheet microscopy with improved FOV for zebrafish intracerebral hemorrhage. Optics express. 30:14709-14722.
Abstract
Airy light-sheet microscopy is rapidly gaining importance for imaging intact biological specimens because of the rapid speed, high resolution, and wide field nature of the imaging method. However, the depth of field (DOF) of the detection objective imposes limitations on the modulation transfer function (MTF) of the light sheet, which in turn affects the size of the field of view (FOV). Here we present an optimized phase modulation model, based on 'Airy-like' beam family, to stretch the curved lobes, which brings a wider FOV while maintaining high resolution. In addition, we further develop a planar 'Airy-like' light-sheet by two-photon excitation which can avoid the deconvolution process. We validated the new imaging method by performing a real-time monitoring of the dynamic process of cerebral hemorrhage in zebrafish larva. The proposed Airy-like beam-based light-sheet microscopy has great potential to be applied to the precise screening of cerebral hemorrhage-related drugs to help precision medicine in the future.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping