FIGURE SUMMARY
Title

Evaluation of the Anticancer Activities of Novel Transition Metal Complexes with Berenil and Nitroimidazole

Authors
Czarnomysy, R., Radomska, D., Muszy?ska, A., Hermanowicz, J.M., Prokop, I., Bielawska, A., Bielawski, K.
Source
Full text @ Molecules
Figure 1

Structures of novel transition metal complexes (Au, Pd, Pt) with berenil and 2-(1-methyl-5-nitroimidazol-2-yl)ethanol.

Figure 2

Viability of MCF-7 (A) and MDA-MB-231 (B) breast cancer cells and normal human breast epithelial cell MCF-10A (C) treated for 24 h with different concentrations of the tested compounds: AuMet-1, PdMet-1, PtMet-1 and cisplatin. Mean values ±SD from 3 independent experiments (n = 3) done in duplicate are presented.

Figure 3

Flow cytometry analysis of MCF-7 (A) and MDA-MB-231 (B) breast cancer cells after 24-h incubation with AuMet-1, PdMet-1, PtMet-1 and cisplatin (50 ?M) and subsequent staining with Annexin V and propidium iodide. Mean percentage values from 3 independent experiments (n = 3) done in duplicate are presented. *p < 0.05 vs. control group, ** p < 0.01 vs. control group.

Figure 4

Fluorescence of MCF-7 (A) and MDA-MB-231 (B) breast cancer cells treated for 24 h with AuMet-1, PdMet-1, PtMet-1 and cisplatin (50 ?M) incubated with mitochondrial membrane potential probe JC-1. Mean percentage values from three independent experiments (n = 3) done in duplicate are presented. * p < 0.05 vs. control group, ** p < 0.01 vs. control group.

Figure 5

Autophagy induction in MCF-7 (A) and MDA-MB-231 (B) breast cancer cells measured by flow cytometry using Autophagy Probe (right-red histogram) compared to negative control cells (left- gray histogram) after 24-h incubation with AuMet-1, PdMet-1, PtMet-1 and cisplatin (50 µM). Mean percentage values from 3 independent experiments (n = 3) done in duplicate are presented. * p < 0.05 vs. control group, ** p < 0.01 vs. control group, *** p < 0.001 vs. control group.

Figure 6

Anti-topoisomerase IIα Antibody flow cytometric analysis of MCF-7 (A) and MDA-MB-231 breast cancer cells (B) (right histogram- green color) compared to a negative control cell (left histogram- gray color) after 24 h of incubation with AuMet-1, PdMet-1, PtMet-1 and cisplatin (50 µM). Mean percentage values from 3 independent experiments (n = 3) done in duplicate are presented. * p < 0.05 vs. control group, ** p < 0.01 vs. control group.
Figure 7

Flow cytometric analysis of DNA fragmentation of MCF-7 (A) and MDA-MB-231 (B) breast cancer cells after 24?h of incubation with AuMet-1, PdMet-1, PtMet-1 and cisplatin (50??M) using TUNEL assay. Histograms present TUNEL negative and TUNEL positive cells. Mean percentage values from 3 independent experiments (n = 3) done in duplicate are presented. * p < 0.05 vs. control group, ** p < 0.01 vs. control group.

Figure 8

Site-specific injection (yolk sac) of transfected (red) breast cancer cells (MCF-7 and MDA-MB-231) into 48 hpf zebrafish embryos and imaging analysis of tumor growth after 48 h of incubation with PdMet-1 and PtMet-1 (100 µM) (A). Quantification of total mCherry fluorescence by breast cancer cells 3 days after injection (B) n = 4, * p < 0.05.

Acknowledgments
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