FIGURE SUMMARY
Title

Elovl2 Is Required for Robust Visual Function in Zebrafish

Authors
Dasyani, M., Gao, F., Xu, Q., Van Fossan, D., Zhang, E., F M Pinto, A., Saghatelian, A., Skowronska-Krawczyk, D., Chao, D.L.
Source
Full text @ Cells
Figure 1

A–C, expression of elovl2 in zebrafish larvae. Lateral view of elovl2 staining in (A) 1 dpf and (B) 2 dpf zebrafish retinas. Lateral (C) and dorsal (C’) view of elovl2 staining in 3 dpf zebrafish retina, respectively. D–F. RNAscope in situ hybridization with elovl2 probe in 7 dpf zebrafish retina showing brightfield (D–F), 4′,6-diamidino-2-phenylindole (DAPI staining (D’–F’), elovl2 (D’’–F’’) and overlay (D’’’–F’’’) images. Scale bars = 50 µm. Magnified view (40×) of boxed regions in Figure 1D’’’. The magnified views of Retina (Panel E) and ciliary marginal zone (CMZ) (Panel F) showing elovl2 staining in 7 dpf zebrafish retina.
Figure 2

Generation and analysis of elovl2 crispants. (A) ω-3 and ω-6 PUFA elongation pathway. (B) Workflow for creating biallelic elovl2 mutants (‘crispants’) in zebrafish using CRISPR/Cas. (C) Significantly changed lipids in elovl2 crispants at days 2 and 7. In particular, all lipids containing DHA are significantly less abundant in elovl2 crispants at both days 2 and 7. (D) Relative abundance of PUFAs in control and elovl2 crispants shows lower abundance of products and elongated products of Elovl2. *** p < 0.005; ns—not significant.
Figure 3

(A) H&E stained retinal sections of elovl2 and control crispants. (B) The thickness of the individual retinal layers of elovl2 and control crispants. Plots showing comparison of lengths of different layers in retina between control and elovl2 crispants. The measurements were taken at three different regions in the retina termed as anterior, posterior, and middle. RGL, Retinal Ganglion Cell Layer; IPL, Inner Plexiform Layer; INL, Inner Nuclear Layer; OPL, Outer Plexiform Layer; ONL, Outer Nuclear Layer; PRL, Photoreceptor Layer. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001, ns—not significant). Each dot is a single cryosection.

PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Days 7-13
Figure 4

(A) Expression of elovl2 RNA and Muller Glia specific gene glula as detected by RNAscope and Glula immunohistochemistry in normal adult zebrafish. (B) Expression of glula in 7dpf control and elovl2 crispants shows no changes of glula expression upon elovl2 knockout.

EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Days 7-13
Figure 5

Zebrafish elovl2 crispants show deficits in the visual motor response (VMF). OFF response activity show visual phenotype. (A) Diagram of motor response (VMR) assay. (B) Motor response of control and elovl2 crispants to VMR-ON (top) and VMR-OFF (bottom) stimulus. Right Charts: quantification of the results at second 1 and 2 after the light stimulus change. AU, arbitrary unit.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cells
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