FIGURE

Figure 2

ID

ZDB-FIG-201229-91

Publication

Dasyani et al., 2020 - Elovl2 Is Required for Robust Visual Function in Zebrafish

Other Figures

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Figure 2

Generation and analysis of elovl2 crispants. (A) ω-3 and ω-6 PUFA elongation pathway. (B) Workflow for creating biallelic elovl2 mutants (‘crispants’) in zebrafish using CRISPR/Cas. (C) Significantly changed lipids in elovl2 crispants at days 2 and 7. In particular, all lipids containing DHA are significantly less abundant in elovl2 crispants at both days 2 and 7. (D) Relative abundance of PUFAs in control and elovl2 crispants shows lower abundance of products and elongated products of Elovl2. *** p < 0.005; ns—not significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + CRISPR3-elovl2 + CRISPR4-elovl2
Knockdown Reagents:	CRISPR3-elovl2 CRISPR4-elovl2
Observed In:	eye very long-chain fatty acid biosynthetic process whole organism very long-chain fatty acid biosynthetic process
Stage Range:	Long-pec to Days 7-13

Phenotype Detail

Acknowledgments

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