FIGURE SUMMARY
Title

Igf2bp1 is required for hepatic outgrowth during early liver development in zebrafish

Authors
Wu, J., Lu, C., Ge, S., Mei, J., Li, X., Guo, W.
Source
Full text @ Gene
Fig. 1

The expression of igf2bp1 during early zebrafish embryo development. (A) Whole-mount in situ hybridization of igf2bp1 from 1-cell to 96hpf. Before 12 hpf and after 72 hpf, the expression of igf2bp1 is low, and igf2bp1 was mainly expressed in brain and liver of zebrafish embryos at 48 hpf and 72 hpf. (B) Igf2bp1 expression was detected by qRT-PCR during embryonic development in zebrafish.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Day 4
Fig. 2

Morpholino-mediated knockdown of igf2bp1. (A) Validation of knockdown efficiency of igf2bp1. Embryos were co-injected with Cont-MO and igf2bp1-GPF (a) or co-injected with igf2bp1-MO1 and igf2bp1-GPF (b). (B) In igf2bp1-MO2, splicing of exon 6 was disrupted with a 59 bp deletion and mRNA translation was prematurely terminated (b), which were indicated by the 633 bp RT-PCR products in Cont-MO and 574 bp in igf2bp1-MO2 (a). (C) No significant developmental malformation was detected in embryos of zebrafish. Cont-MO: Embryos injected with control MO. igf2bp1-MO1/2: Embryos injected with igf2bp1-MO1or igf2bp1-MO2.

PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec
Fig. 3

The effects of igf2bp1 knockdown on liver marker genes. Lateral views of l-fabp10a probe in Cont-MO (A), igf2bp1-MO1/2 (B) and igf2bp1-MO1/2 + 5 bp-mismatched igf2bp1 capped mRNA (C) co-injected embryos at 72 hpf.

EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth
Fig. 4

Igf2bp1 is required for hepatic outgrowth but not for hepatoblast specification and budding. Whole-mount in situ hybridization of the early endoderm marker gene expression in Cont-MO (A, C, E and G) and igf2bp1-MO1/2 (B, D, F and H) embryos at 30 and 48 hpf.

EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Long-pec
Fig. 6

The effects of igf2bp1 knockout on liver marker genes. (A) No significant developmental malformation was found in igf2bp1Δ5 mutant. (B) Whole-mount in situ hybridization showed the expression of early endoderm marker genes in wild-type (a, c, e and g) and igf2bp1Δ5 mutants (b, d, f and h). (C) Whole-mount in situ hybridization with l-fabp10a probe in wild-type (a) and igf2bp1Δ5 mutants (b).

Fig. 7

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. s1

Supplementary figure 1.

text only

Unillustrated author statements

PHENOTYPE:
Fish:
Observed In:
Stage: Adult
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Gene, 744, Wu, J., Lu, C., Ge, S., Mei, J., Li, X., Guo, W., Igf2bp1 is required for hepatic outgrowth during early liver development in zebrafish, 144632, Copyright (2020) with permission from Elsevier. Full text @ Gene
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.