FIGURE

Fig. 2

ID

ZDB-FIG-200518-33

Publication

Wu et al., 2020 - Igf2bp1 is required for hepatic outgrowth during early liver development in zebrafish

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Fig. 2

Morpholino-mediated knockdown of igf2bp1. (A) Validation of knockdown efficiency of igf2bp1. Embryos were co-injected with Cont-MO and igf2bp1-GPF (a) or co-injected with igf2bp1-MO1 and igf2bp1-GPF (b). (B) In igf2bp1-MO2, splicing of exon 6 was disrupted with a 59 bp deletion and mRNA translation was prematurely terminated (b), which were indicated by the 633 bp RT-PCR products in Cont-MO and 574 bp in igf2bp1-MO2 (a). (C) No significant developmental malformation was detected in embryos of zebrafish. Cont-MO: Embryos injected with control MO. igf2bp1-MO1/2: Embryos injected with igf2bp1-MO1or igf2bp1-MO2.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO3-igf2bp1 + MO4-igf2bp1
Knockdown Reagents:	MO3-igf2bp1 MO4-igf2bp1
Observed In:	whole organism
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Gene, 744, Wu, J., Lu, C., Ge, S., Mei, J., Li, X., Guo, W., Igf2bp1 is required for hepatic outgrowth during early liver development in zebrafish, 144632, Copyright (2020) with permission from Elsevier. Full text @ Gene