PUBLICATION
Igf2bp1 is required for hepatic outgrowth during early liver development in zebrafish
- Authors
- Wu, J., Lu, C., Ge, S., Mei, J., Li, X., Guo, W.
- ID
- ZDB-PUB-200403-248
- Date
- 2020
- Source
- Gene 744: 144632 (Journal)
- Registered Authors
- Keywords
- CRISPR/Cas9, igf2bp1, liver development, morpholino, zebrafish
- MeSH Terms
-
- Animals
- Cell Proliferation
- Hepatocytes/cytology
- Liver/anatomy & histology
- Liver/embryology*
- Liver/metabolism
- Morpholinos
- Organ Size
- Phenotype
- RNA-Binding Proteins/antagonists & inhibitors
- RNA-Binding Proteins/genetics
- RNA-Binding Proteins/metabolism
- RNA-Binding Proteins/physiology*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- PubMed
- 32240777 Full text @ Gene
Citation
Wu, J., Lu, C., Ge, S., Mei, J., Li, X., Guo, W. (2020) Igf2bp1 is required for hepatic outgrowth during early liver development in zebrafish. Gene. 744:144632.
Abstract
IGF2BPs, a subclass of RNA-binding proteins, regulate cellular differentiation, proliferation and migration during multiple organs development, but their functions in liver development still remain unclear. Here, in this study, whole-mount in situ hybridization showed that igf2bp1 was constantly and stably expressed at early stages of embryo development in zebrafish. Both the morpholino-induced knockdown and CRISPR/Cas9-mediated knockout of igf2bp1 led to a reduced-size liver phenotype. Further analysis revealed that igf2bp1 is required for hepatic outgrowth, but not for hepatoblast specification and budding. Deficiency of igf2bp1 resulted in reduced cell proliferation, but had no effect on apoptosis. Therefore, we concluded that igf2bp1 is a critical factor to regulate hepatic outgrowth via cell proliferation during early liver development in zebrafish.
Errata / Notes
This article is corrected by ZDB-PUB-220920-26.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping