The Jade-2-LSD1 Pathway Is Implicated in Nervous System Development

(A) The indicated electroporated cortical slices were analyzed by IF microscopy for βIII-tubulin or Nestin. The images of VZs and/or IZs are shown. Scale bar represents 20 μm. The percentage of GFP⁺/βIII-tubulin⁺, or GFP⁺/Nestin⁺ cells to GFP⁺ cells was quantified by ImageJ. Slices from two independent experiments were processed for each experimental condition. The results are mean ± SEM of three sections from each experiment. ^∗p < 0.05 and ^∗∗p < 0.01 (two-tailed unpaired Student’s t test). The images of “shCTR” refer to that in Figure 1E, given that the experiments with shLsd1, shJade-2, or shJade-2⁺shLsd1 were compared to the same control.

(B) Ten nanograms of each of the indicated morpholinos were injected into one-cell stage embryos before the mRNA level of sox2, sox3, otx2, or hoxb1b was examined at 75% epiboly stage by whole-mount in situ hybridization. Lateral view of dorsal to the right is shown. Scale bar represents 200 μm. The number of observed live embryos (n) was quantified and the ratio of the affected embryos is shown.

See also Figure S4.

Related to Figure 6.The efficiency of phf15 morpholino (MO)or kdm1a MO.One-hundred pg of phf15-5'UTR-EGFP plasmids were injected or co-injected with 10ng of phf15 MOsinto one-cell stage zebrafish embryos. The embryos were then observed by phase-contrast microscopy or analyzed for GFP by immunofluorescence microscopy at shield stage. Alternatively,50 pg of kdm1a-5'UTR-EGFP plasmids were injected or co-injected with 10ng of kdm1aMOs into one-cell stage embryos before the embryos were observed by phase-contrast microscopy or analyzed for GFP by immunofluorescence microscopy at shield stage.Scale bar: 200 µm