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Fig. S4
Related to Figure 6.The efficiency of phf15 morpholino (MO)or kdm1a MO.One-hundred pg of phf15-5'UTR-EGFP plasmids were injected or co-injected with 10ng of phf15 MOsinto one-cell stage zebrafish embryos. The embryos were then observed by phase-contrast microscopy or analyzed for GFP by immunofluorescence microscopy at shield stage. Alternatively,50 pg of kdm1a-5'UTR-EGFP plasmids were injected or co-injected with 10ng of kdm1aMOs into one-cell stage embryos before the embryos were observed by phase-contrast microscopy or analyzed for GFP by immunofluorescence microscopy at shield stage.Scale bar: 200 µm
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