- Title
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A Forward Genetic Screen Targeting the Endothelium Reveals a Regulatory Role for the Lipid Kinase Pi4ka in Myelo- and Erythropoiesis
- Authors
- Ziyad, S., Riordan, J.D., Cavanaugh, A.M., Su, T., Hernandez, G.E., Hilfenhaus, G., Morselli, M., Huynh, K., Wang, K., Chen, J.N., Dupuy, A.J., Iruela-Arispe, M.L.
- Source
- Full text @ Cell Rep.
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(A) Morpholino targeted splicing in the catalytic domain. PCR to access splicing efficiency. (B) O-dianisidine marking hemoglobin in control and morphant fish. (C) 24 hpf control injected embryos were subjected to flow cytometry and Gata1:DsRED+ was plotted against Fli1:GFP (left). Histogram of Gata1:DsRED+ cells as a subpopulation of Fli1:GFP cell population. (D,E) Identical gating performed on 48 hour control (top) and morphant embryos (bottom). (F) G1E-ER4 cell differentiation in the prescence of 4-hydroxytamoxifen (solid line) or ethenal control (dashed line). Cells were either pre-treated with non-targeted shRNA (grey) or Pi4ka targeted shRNA (red). Median fluorescence intensity (MFIR) was calculated as a ratio of MFI relative to shScrmb control. Statistical t-test comparing 3 independent experiments. (* p<0.05, **p<0.01, ***p<0.001, ****p<0.0001) PHENOTYPE:
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