Transgenic Construct

Tg(kop:EGFP-FTASE-UTR-nanos3)

ID

ZDB-TGCONSTRCT-070406-1

Previous Names

Tg(kop:EGFP-UTR-nanos3)
Tg(kop:EGFP-UTRnanos)
Tg(kop:EGFP-UTRnanos3)

Type

engineered_plasmid

Regulatory Regions

kop (1)

Coding Sequences

EGFP (1)
FTASE (1)
nanos3 (1)

[Em λ][Ex λ]

Fpbase:EGFP

Contains Sequences

UTR (1)

Note

The kop promoter was cloned upstream of EGFP containing a farnesylation signal, followed by the 3' UTR of the nanos gene. The construct was cloned into the pTol2000 vector. The transgene directs EGFP expression in the plasma membrane of primordial germ cells.

Plasmid Map

None

Genomic Features

That Utilize Tg(kop:EGFP-FTASE-UTR-nanos3) Construct

Genomic Feature	Affected Genomic Regions
bjz2Tg
er1Tg

Transgenics

That Utilize Tg(kop:EGFP-FTASE-UTR-nanos3) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
cxcl12a^t30516/+; er1Tg	cxcl12a		Fig. 3 from Tarbashevich et al., 2015
cxcl12a^t30516/+; er1Tg + MO1-ca15b		Fig. 3 from Tarbashevich et al., 2015	Fig. 3 from Tarbashevich et al., 2015
er1Tg		2 figures from 2 publications	10 figures from 7 publications
er1Tg (AB)
er1Tg + CRISPR1-plpp1a + CRISPR1-plpp1l + CRISPR1-plpp2a + CRISPR1-plpp2b + CRISPR1-plpp3 + CRISPR1-ppap2d + CRISPR2-plpp1a + CRISPR2-plpp1l + CRISPR2-plpp2a + CRISPR2-plpp2b + CRISPR2-plpp3 + CRISPR2-ppap2d + CRISPR3-plpp1a + CRISPR3-plpp1l + CRISPR3-plpp2a + CRISPR3-plpp2b + CRISPR3-plpp3 + CRISPR3-ppap2d + CRISPR4-plpp1a + CRISPR4-plpp1l + CRISPR4-plpp2a + CRISPR4-plpp2b + CRISPR4-plpp3 + CRISPR4-ppap2d + CRISPR5-plpp1a		Fig. 3 from Paksa et al., 2016	Fig. 3 from Paksa et al., 2016
er1Tg + CRISPR21-tyr + CRISPR22-tyr + CRISPR23-tyr + CRISPR24-tyr + CRISPR3-slc45a2 + CRISPR4-slc45a2 + CRISPR5-slc24a5 + CRISPR5-slc45a2 + CRISPR6-slc24a5 + CRISPR6-slc45a2 + CRISPR7-slc24a5 + CRISPR8-slc24a5			Fig. 3 from Paksa et al., 2016
er1Tg + MO1-ca15b		Fig. 3 from Tarbashevich et al., 2015	Fig. 3 from Tarbashevich et al., 2015
er1Tg + MO1-rgs14a		Fig. 3 from Hartwig et al., 2014	Fig. 3 from Hartwig et al., 2014
er1Tg + MO3-ackr3b		Fig. 4 from Boldajipour et al., 2008	Fig. 4 from Boldajipour et al., 2008
er1Tg + MO3-cxcl12a		Fig. 4 from Boldajipour et al., 2008	Fig. 4 from Boldajipour et al., 2008
er1Tg; mu4Tg			Fig. 4 from Tarbashevich et al., 2015
er1Tg; mu4Tg + MO1-ca15b		Fig. 4 from Tarbashevich et al., 2015	Fig. 4 from Tarbashevich et al., 2015
paf1^z24/z24; er1Tg	paf1		Fig. 5 from Jurynec et al., 2019
tdrd6a^mz0d6d7/+; er1Tg	tdrd6a		Fig. 2 from Roovers et al., 2018

Sequence Information

Other Construct Pages

None

Citations